款冬花多糖的羧甲基化衍生物合成工艺研究  

Study on Synthetic Conditions of Carboxymethylated Derivatives of Polysaccharides from Tussilago farfara L.

作　　者：张丽华[1] 宋逍[1] 赵鹏[1] 唐志书[1] 李稳宏[2] 

机构地区：[1]陕西中医学院药学院,咸阳712046 [2]西北大学化工学院,西安710069

出　　处：《天然产物研究与开发》2013年第7期892-896,共5页Natural Product Research and Development

基　　金：陕西省自然科学基金项目(2011JQ2005);陕西省科学技术研究发展计划(2012K19-04-07);陕西省教育厅自然科学专项项目(2013JK0764)

摘　　要：本文应用响应面法优化制备款冬花多糖羧甲基化衍生物的合成工艺条件。采用碱性条件的氯乙酸法,以羧甲基的取代度为考察指标,最佳合成工艺为:氯乙酸浓度为2.5 mol/L,反应温度52℃,反应时间3.1 h。羧甲基化后的多糖清除DPPH的能力提高,抗氧化性提高,为今后多糖衍生物的产业化工艺开发有较好的参考价值和依据。Polysaccharides from Tussilago farfara L （TFPs） was reacted with the chloroacetic acid in alkaline condi- tions. According to the degree of substitution, Response Surface Methodology was applied to optimize the carboxymethyl modification. The effects of carboxymethyl Tussilagofarfara L. polysaccharides （CTFPs） on scavenging DPPH was also determined. The results showed that 2.5 mol/L of chloroacetic acid, react for 3.1 h under 52 ℃ were the optimum syn- thetic conditions. Compared with TFPs,the effect of CTFPs on scavenging DPPH was improved.

关 键 词：款冬花 多糖 羧甲基化 抗氧化性 

分 类 号：O629.12[理学—有机化学]