3株产气内生真菌的水果防腐活性及其气体成分分析  

Activity of Three Volatile-Producing Endophytic Muscodor Strains in Controlling Fruit Postharvest Disease and Analysis of the Volatile Components

作　　者：袁志林[1] 陈益存[1] 毛黎娟[2] 章初龙[3] 陈连庆[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,富阳311400 [2]浙江大学农生环分析测试中心,杭州310058 [3]浙江大学生物技术研究所水稻生物学国家重点实验室,杭州310058

出　　处：《林业科学》2013年第6期83-89,共7页Scientia Silvae Sinicae

基　　金：浙江省科技厅公益技术研究农业项目(2011C22045);中央级公益性科研院所基本科研业务专项资金(RISF6902);国家高技术研究发展计划(863计划)课题(2011AA10A205-03)

摘　　要：从极端濒危植物百山祖冷杉针叶组织中分离到3株属于炭角菌科产气霉属的内生菌,这些菌株能产生强烈的挥发性物质,对多种植物病原菌具有明显的抑制或致死功能;水果保鲜试验表明固体发酵菌剂能有效防治草莓灰葡萄孢霉、苹果轮纹病菌和油桃褐腐病菌等引起的水果采后病害。固相微萃取和气质联用(SPME-GC-MS)分析表明,3个菌株产生的气体成分及含量存在较大差异,M25成分单一,而M112-2和M153成分较复杂,这种差异性与其生物学活性有一定的相关性。主要气体物质为二甲酯丙酸、甲基-2-甲基丙酸酯和β-水芹烯等。Three endophytic Muscodor isolates isolated from needle tissues of the critically threatened Abies beshanzuensis can produce intense volatiles in vitro, which in general cause obvious inhibition or even death to a wide range of plant pathogens, indicating their strong antibiotic activity. In this paper, the fungal agents derived from solid- state fermentation were applied to efficiently control the fruit postharvest diseases, including Botrytis cinerea, Physalospora pyricola and Moniliniafructicola. The SPME-GC-MS technique was used to detect the volatile profiling of Mucodor, and the result showed that there were obvious difference in the chemical components and relative content among the three Mucodor isolates. Muscodor sp. M25 had relatively simple chemical composition of volatiles, while Muscodorfengyangensis Ml12-2 and M153 had complicated composition. The difference in their chemical composition of volatiles was, to some degree, correlated with their bioactivity. Propanoic acid, 2-methyl-, propanoic acid, 2-methyl-, methyl ester and [3- phellandrene were the main volatile small molecules. Thus, those Muscodor isolates would be ideal candidates to be exploited as novel ＇ myco-fumigation＇ agents.

关 键 词：产气霉 固相微萃取 抑菌活性 

分 类 号：S763.15[农业科学—森林保护学]