油茶EMF2基因的全长cDNA克隆及序列分析  

Cloning and sequence analysis of full-length cDNA of EMF2 gene in Camellia oleifera

作　　者：邬萌萌[1] 谭晓风[2] 周荣[1] 詹文勇 胡孝义[1] 

机构地区：[1]安徽农业大学林学与园林学院,安徽合肥230036 [2]中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙410004 [3]安徽省油茶良种工程技术研究中心,安徽舒城231340

出　　处：《经济林研究》2013年第2期7-12,共6页Non-wood Forest Research

基　　金：国家自然科学基金项目"用树体环剥技术快速分析油茶基因功能的方法研究"(31070605)

摘　　要：EMF2基因是植物营养组织中的主要开花抑制基因,在植物花器官发育过程中发挥重要的作用。为给油茶性状表观遗传学调控研究提供参考依据,以"华鑫"油茶的组培幼苗为试验材料,采用3′RACE和5′RACE技术对油茶EMF2基因进行克隆及序列分析。结果表明:该基因全长2 210 bp,其中开放阅读框为1 866 bp,编码621个氨基酸,该基因被命名为Co-EMF2。多序列比对后发现,Co-EMF2具有EMF2基因特有的保守序列和相关特征,且与其它植物的EMF2基因具有较高的相似性。文中进而对其蛋白质序列和理化性质进行了分析,结果表明:其蛋白质属不稳定蛋白,等电点为6.17,是疏水性蛋白,亚细胞定位预测结果表明其位于细胞核中。EMF2 gene maintains vegetative development by repressing flowering program, and plays an important role in the process of flower organ development. In order to offer a reference for the researches on epigenetic regulation of Camellia oleifera characters, the full-length eDNA of EMF2 gene in C. oleifera （Huaxin） was cloned and analyzed by 5＇RACE and 3＇RACE techniques. The results show that the full length of the gene is 2 210 bp, the ORF box included 1 866 bp and encodes 621 amino acids, and it is named Co-EMF2. The results of multiple sequence alignment show that Co-EMF2 has the typical conserved sequences of EMF2 and relevant characters, and it has high homology with EMF2 in other plant species, the protein is a labile and hydrophobic nuclear protein, and its lsoelectric point is 6.17.

关 键 词：油茶 EMF2基因 基因克隆 全长CDNA 序列分析 

分 类 号：S603.2[农业科学—园艺学] S794.4