痘苗病毒真核表达载体pM J601的转化与鉴定  

Transformation and Identification of Vaccinia Expression Vector pMJ601

作　　者：郜恒骏[1] 朱红音[1] 楼屹[1] 顾伟齐 房静远 任卫平 萧树东[1] 

机构地区：[1]上海第二医科大学仁济医院消化科,上海市消化疾病研究所,上海200001

出　　处：《南京医科大学学报（自然科学版）》2000年第6期481-483,共3页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金资助项目 ( 3 9770 3 4 5 )

摘　　要：目的　对痘苗病毒表达载体 p MJ6 0 1进行转化与鉴定 ,为进一步实施痘苗病毒为载体的基因治疗作必要的准备。方法　利用感受态细胞的制备及转化、质粒抽提、琼脂糖凝胶电泳、限制性内切酶酶切等多种基因工程技术对质粒 p MJ6 0 1进行转化及鉴定。结果　琼脂糖凝胶电泳清楚地显示了 p MJ6 0 1质粒 3条带和 Bam HI或 H ind 酶切后的线性条带。结论　痘苗病毒表达载体 p MJ6 0 1的转化与鉴定 ,为痘苗病毒载体系统的基因治疗打下了坚实的基础。Objective To transform and identify vaccinia expression vector, pMJ601, for the necessary equipment of gene therapy based on the vector of pox virus. Methods Genetic engineering techniques such as preparation and transformation of competent cell, plasmid extraction, agarose gel electrophoresis, restriction analysis, and some others were used to transform and identify pox virus expression vector pMJ601.Results There were three fragments in pMJ601, which were apparently shown by agarose gel electrophoresis.Conclusion It is one of the critical steps to further gene therapy based on pox virus.

关 键 词：痘苗病毒 表达载体 pMJ601 基因工程 真核 

分 类 号：R394.8[医药卫生—医学遗传学] Q813.6[医药卫生—基础医学]