地瓜叶总黄酮的超声辅助提取及生物活性研究  

Ultrasonic-Assisted Extraction and Bioactivity of Flavonoids from Ipomoea batatas Lam Leaves

作　　者：许瑞波[1] 周洪英[1] 陈林[1] 曾艳霞[1] 杨树平[1] 胡喜兰[1] 

机构地区：[1]淮海工学院化工学院,江苏连云港222005

出　　处：《食品科学》2013年第14期141-146,共6页Food Science

基　　金：连云港市科技攻关项目(CG1132);淮海工学院博士科研启动项目(KQ10130)

摘　　要：以地瓜叶为原料,采用超声波辅助的方法,通过单因素和正交试验优化红薯叶总黄酮(FIBL)的提取工艺条件,并且研究了FIBL的体外抗氧化活性和抑菌作用。结果表明,从地瓜叶中提取总黄酮的最佳工艺条件为乙醇体积分数75%、液料比30:1(mL/g)、超声提取40min,然后70℃水浴加热回流提取50min,提取2次,提取率为6.03%。用紫外光谱和红外光谱对提取产物进行表征,证明产物FIBL具备黄酮类化合物的基本光谱特征。此外,抗氧化实验结果表明,FIBL对超氧阴离子自由基和DPPH自由基具有一定的清除能力,但不如VC的清除效果好。抑菌实验结果表明,FIBL对金黄色葡萄球菌、大肠杆菌和枯草芽孢杆菌具有良好的抑制作用。The ultrasonic-assisted extraction of flavonoids from Ipomoea batatas Lam leaves（FIBL） was explored by orthogonal array design.The antioxidant and bacteriostatic activities of FIBL in vitro were investigated.The results showed that the optimal extraction conditions of FIBL were ethanol concentration of 75%,liquid-solid of 30：1（mL/g）,ultrasonic treatment time of 40 min,reflux extraction time of 50 min,extraction temperature of 70 ℃,and repeated extraction time of 2.Under the optimal extraction conditions,the yield of FIBL was up to 6.03%.As characterized by UV-vis and IR spectroscopy,FIBL possessed common spectral characteristics of flavonoids.Furthermore,FIBL had scavenging capacity against superoxide anion and DPPH radicals,but weaker than vitamin C.FIBL also revealed excellent inhibitory effect on Staphylococcus aureus,Escherichia coli and Bacillus subtilis.

关 键 词：地瓜叶 黄酮类化合物 提取 抗氧化活性 抑菌作用 

分 类 号：R284.2[医药卫生—中药学]