Atomic force microscopy imaging of live mammalian cells  

作　　者：LI Mi LIU LianQing XI Ning WANG YueChao DONG ZaiLi XIAO XiuBin ZHANG WeiJing 

机构地区：[1]State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences [2]University of Chinese Academy of Sciences [3]Department of Mechanical and Biomedical Engineering, City University of Hong Kong [4]Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences

出　　处：《Science China(Life Sciences)》2013年第9期811-817,共7页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (61175103);CAS FEA International Partnership Program for Creative Research Teams

摘　　要：Atomic force microscopy (AFM) was used to examine the morphology of live mammalian adherent and suspended cells. Time-lapse AFM was used to record the locomotion dynamics of MCF-7 and Neuro-2a cells. When a MCF-7 cell retracted, many small sawtooth-like filopodia formed and reorganized, and the thickness of cellular lamellipodium increased as the retraction progressed. In elongated Neuro-2a cells, the cytoskeleton reorganized from an irregular to a parallel, linear morphology. Suspended mammalian cells were immobilized by method combining polydimethylsiloxane-fabricated wells with poly-L-lysine electrostatic adsorption. In this way, the morphology of a single live lymphoma cell was imaged by AFM. The experimental results can improve our understanding of cell locomotion and may lead to improved immobilization strategies.Atomic force microscopy （AFM） was used to examine the morphology of live mammalian adherent and suspended cells. Time-lapse AFM was used to record the locomotion dynamics of MCF-7 and Neuro-2a cells. When a MCF-7 cell retracted, many small sawtooth-like filopodia formed and reorganized, and the thickness of cellular lamellipodium increased as the retraction progressed. In elongated Neuro-2a cells, the cytoskeleton reorganized from an irregular to a parallel, linear morphology. Suspended mammalian cells were immobilized by method combining polydimethylsiloxane-fabricated wells with poly-L-lysine electrostatic adsorption. In this way, the morphology of a single live lymphoma cell was imaged by AFM. The experimental results can improve our understanding of cell locomotion and may lead to improved immobilization strategies.

关 键 词：atomic force microscopy cell locomotion LAMELLIPODIA CYTOSKELETON POLYDIMETHYLSILOXANE 

分 类 号：Q952[生物学—动物学] TH742[机械工程—光学工程]