一株细菌纤维素生产菌的分离鉴定  

Isolation and identification on high-producing bacteria of bacterial cellulose

作　　者：卢美欢[1] 马英辉[1] 王银存[1,2] 王小娟[1,2] 李利军[1] 

机构地区：[1]陕西省微生物研究所,陕西西安710043 [2]西北大学生命科学学院,陕西西安710069

出　　处：《中国酿造》2013年第7期46-49,共4页China Brewing

基　　金：陕西省科学院青年人才基金(2010k-29)

摘　　要：获得一株细菌纤维素高产菌株并对其进行鉴定。从自制醋醅、红茶菌液及残次水果等材料中筛选细菌纤维素高产菌株。采用生理生化鉴定结合16S rDNA序列的系统发育学分析确定该菌株的系统发育地位。获得的菌株AXB(X)为好氧性的革兰氏阴性杆菌,菌体大小为(0.6μm^0.8μm)×(1.5μm^2.0μm),单个、成对或成链。菌株AXB(X)与Gluconacetobacter sp.的16S rDNA序列同源性为98%。经鉴定菌株AXB(X)为葡糖杆菌属。To obtain a high-yield strain of bacterial cellulose and its identification. High-yield strains of the bacterial cellulose were screened from the vinegar grains, kombucha liquid, defective fruits, and other materials. Physiological and biochemical identification was combined with 16S rDNA se- quence phylogenetic analysis to determine the phylogenetic position of the strain. Strain AXB （X） is the aerobic gram-negative bacilli, and the cell size is （0.6μm-0.8μm）× （1.5μm-2.0μm）, single, in pairs or into a chain. 16S rDNA sequence of strain AXB （X） and Gluconacetobacter sp.sequence ho- mology was up to 98%. After initial determine, strain AXB（X） was found as a Gluconacetobacter sp.

关 键 词：细菌纤维素 鉴定 葡糖杆菌属 

分 类 号：Q93-331[生物学—微生物学]