高效液相色谱-蒸发光散射法测定食品中的单糖、双糖、低聚果糖和糖醇  

Simultaneous determination of monosaccharides,disaccharides, oligosaccharides and sugar alcohols in foods by high performance liquid chromatography with evaporative light-scattering detection

作　　者：丁洪流[1] 李灿 金萍[1] 袁丽红 姚永青[1] 陈英[1] 李培[1] 

机构地区：[1]苏州市产品质量监督检验所,江苏苏州215000 [2]江南大学,江苏无锡214000

出　　处：《色谱》2013年第8期804-808,共5页Chinese Journal of Chromatography

基　　金：国家质检总局科技计划项目(2010QK179)

摘　　要：建立了食品中常用的木糖、果糖、葡萄糖、蔗糖、麦芽糖、乳糖、蔗果三糖、蔗果四糖、蔗果五糖、赤藓糖醇、木糖醇、甘露糖醇、麦芽糖醇等13种单糖、双糖、低聚果糖和糖醇的高效液相色谱同时分离检测的方法。该法采用NH2色谱柱,以乙腈-水为流动相梯度洗脱,蒸发光散射检测器检测;13种糖在0.1～5 g/L内均具有良好的线性关系,检出限均在0.1 g/L以下,精密度(RSD)为2.69%～7.21%,回收率为96.1%～105.2%,结果较为理想。将该法用于实际样品检测,结果显示食品标签明示和实际成分相差较大。A simple and efficient method was established based on high performance liquid chromatography （HPLC） to separate and detect thirteen analytes of monosaccharides, disac- cbarides, oligosaccharides and sugar alcohols （xylose, fructose, glucose, sucrose, maltose, lactose, l-kestose, nystose, I F-fructofuranosyl nystose, erythritol, mannitol, xylitol, maltitol） in foods. The separation was performed on an NH2 column with the gradient elution of acetoni- trile-water as the mobile phases. The analytes were detected by an evaporative light-scattering detector （ELSD）. All the thirteen sugars had good linearities within 0. l - 5 g/L with the corre- lation coefficients between 0. 990 1 - 0. 999 6. The limits of detection （LOD） were all less than 0. l g/L. The precisions of the method expressed as RSDs were in the range of 2.59% -7.21%. The recoveries of the thirteen analytes spiked in real samples ranged from 95. 1% to 105.2%. This method was applied to the actual sample testings and the results showed the food labels were greatly different from the actual compositions .

关 键 词：高效液相色谱 蒸发光散射检测 糖 糖醇 食品 

分 类 号：O658[理学—分析化学]