应用PCR-SSCP非同位素技术检测人卵巢癌nm_(23)-H_1基因突变的研究  

The mutations of nm_(23)-H_1 associated with malignancy of ovarian tumors

作　　者：王忠民[1] 常德安[2] 

机构地区：[1]大连市妇产医院,辽宁大连116033 [2]大连医科大学第二临床学院,辽宁大连116027

出　　处：《大连医科大学学报》2000年第3期161-164,共4页Journal of Dalian Medical University

基　　金：大连市卫生局科研计划资助课题!9948号

摘　　要：目的 :研究卵巢癌中是否存在nm2 3-H1基因突变及其与卵巢癌分期、组织学类型、分化程度的关系 ;方法 :利用PCR -SSCP技术研究了正常卵巢、良性及恶性卵巢瘤中nm2 3-H1基因的 5个外显子的突变情况 ;结果 :正常卵巢、良性及交界性卵巢肿瘤未见突变 ,32例恶性卵巢瘤中 9例有突变 ;低分化程度卵巢癌的突变率高于高、中分化程度的卵巢癌 (P <0 0 0 5) ;浆液性卵巢癌的突变率高于其他组织学类型的卵巢癌 (P <0 0 0 5) ;Ⅲ、Ⅳ期突变率与Ⅰ、Ⅱ期之间相比有显著性差别 (P <0 0 5) ;有淋巴结转移的卵巢癌基因突变率高于无淋巴结转移卵巢癌(P <0 0 5) ;结论 :nm2 3-H1基因突变易发生于分化程度较低。Objective:To find out the relationship of mutations of nm 23 -H 1 to stages,histological types and differentiation of ovarian tumors.Method:The polymerase chain reaction-single strand conformational polymorphism(PCR-SSCP)analysis was performed to examine mutations in 5 exons of nm 23 -H 1 gene in samples from normal ovaries,benign and malignant ovarian tumors.Results:Mutation was not found in normal ovaries,benign and borderline ovarian tumors,while was found in 9 out of 32 cases of malignant ovarian tumors( P <0 05).The mutation rate of serous carcinoma was higher than mucinous carcinoma,endometrioid carcinoma and clear cell carcinoma( P <0 005).The mutation rate of carcinomas with stage Ⅲ or Ⅳ were higher than those with stage I or Ⅱ( P <0 05).The mutation rate was higher in cases with lymph node metastases than those without lymph node metastases( P <0 05).Conclusion:These results suggested that mutation of nm 23 -H 1 gene tend to exist in advanced serous ovarian carcinomas with lymph node metastases.

关 键 词：卵巢癌 NM23-H1基因 PCR-SSCP 基因突变 

分 类 号：R737.31[医药卫生—肿瘤]