正交试验优化大孔吸附树脂分离纯化杜仲绿原酸  

Optimization by Orthogonal Array Design of Separation and Purification of Chlorogenic Acid from Eucommia ulmoidues Leaves by Macroporous Adsorption Resin

作　　者：熊硕[1] 崔丽[1] 刘仲华[1] 张盛[1] 肖文军[1] 

机构地区：[1]湖南农业大学园艺园林学院,国家植物功能成分工程技术研究中心,湖南长沙410128

出　　处：《食品科学》2013年第16期23-26,共4页Food Science

基　　金："十二五"国家科技支撑计划项目(2011BAD10B00);国家农业科技成果转化资金项目(2011GB2D200010)

摘　　要：以杜仲叶60%乙醇提取液为原料,研究NKA-2、NKA-9、LS-600、HZ806、HZ906、HPD100、HPD500、S-8八种大孔吸附树脂对绿原酸的静态吸附与解吸效果,优化最优树脂分离纯化杜仲叶绿原酸的工艺技术参数。结果表明:NKA-2树脂对绿原酸具有较好的吸附与解吸性能,在pH3的条件下静态吸附量为86mg/g,解吸率达93.23%;当主要考虑绿原酸得率时,最优工艺参数为上样质量浓度4mg/mL、上样流速4BV/h、上样体积5BV、洗脱剂乙醇体积分数80%、洗脱流速为3BV/h、洗脱体积为2BV,在该工艺参数条件下,绿原酸得率为83.14%,纯度为28.91%;当主要考虑绿原酸纯度时,最优工艺参数为上样质量浓度4mg/mL、上样流速4BV/h、上样体积5BV、洗脱剂乙醇体积分数80%、洗脱流速为3BV/h、洗脱体积为1.5BV,在该工艺参数条件下,绿原酸得率为79.62%,纯度为39.38%。Different types of macroporous adsorption resin,including NKA-2,NKA-9,LS-600,HZ806,HZ906,HPD100,HPD500 and S-8 were compared for their static adsorption and desorption capacities for chlorogenic acid in 60% ethanol extract of Eucommiaulmoidues leaves.NKA-2 was found to have a stronger ability to adsorb and desorb chlorogenic acid and the optimal operating parameters for purifying chlorogenic acid from the ethanol extract using NKA-2 were determined.The static adsorption quantity of chlorogenic acid on NKA-2 and desorption percentage were 86 mg/g and 93.23%,respectively.The optimal process conditions that provided maximum chlorogenic acid recovery（83.14%;purity 28.91%） were determined as 4 mg/mL,4 BV/h,5 BV,80%,3 BV/h and 2 BV for sample concentration,loading flow rate,loading volume,desorbent concentration,desorption flow rate,and desorbent volume,respectively,and whereas those for maximum chlorogenic acid purity（39.38%;recovery 79.62%） were 4 mg/mL,4 BV/h,5 BV,80%,3 BV/h and 1.5 BV,respectively.

关 键 词：杜仲叶 绿原酸 大孔吸附树脂 分离纯化 

分 类 号：TQ460.72[医药卫生—药物分析学]