基于石墨烯-壳聚糖修饰电极的免疫传感器检测三聚氰胺  

Electrochemical Immunosensor for Melamine Determination Based on Graphene-Chitosan Composite Film Modified Electrode

作　　者：邵科峰[1] 陈昌云[2] 颜妍[1] 吴珺[1] 赵波[1] 

机构地区：[1]南京师范大学化学与材料科学学院,江苏南京210023 [2]南京晓庄学院生物化工与环境工程学院,江苏南京211171

出　　处：《食品科学》2013年第16期221-225,共5页Food Science

基　　金：上海市科委重点科技攻关项目(10391901800);江苏省科技厅科技支撑计划--工业项目(BE2011196);江苏省农业科技自主创新资金项目(CX123088)

摘　　要：构建一种基于石墨烯-壳聚糖修饰电极的检测三聚氰胺的电化学免疫传感器。利用三聚氰胺抗体和三聚氰胺之间特定反应的竞争模式,以K3Fe(CN)6为探针,通过循环伏安法和差分脉冲伏安法监测免疫反应,对溶液中的三聚氰胺进行检测。检测线性范围宽(5～1500ng/mL),检出限可低至0.2ng/mL。同时,对牛奶实际样品进行测定,回收率为104.0%～106.2%。An electrochemical immunosensor for the determination of melamine based on graphene-chitosan modified electrode was constructed.Indirect competitive electrochemical immunoassay was performed to determine melamine in aqueous solutions.Cyclic voltammetry（CV） and differential pulse voltammetry（DPV） were carried out to monitor the fabrication steps of immunoreaction system in solutions containing K3Fe（CN）6 as redox probe.The immunosensor demonstrated excellent melamine detection features with a broad linear range of 5 to 1500 ng/mL and a detection limit of 0.2 ng/mL.A good recovery of melamine in the range of 104%–106.2% was obtained in spiked milk samples.

关 键 词：石墨烯 壳聚糖 三聚氰胺 电化学免疫传感器 

分 类 号：TS202.3[轻工技术与工程—食品科学]