高效液相色谱-串联质谱联用技术鉴定樱桃叶中的黄酮成分  

Identification of Chemical Constituents of Flavonoids in the Leaves of Prunuspseudocerasus L. by HPLC-MS-MS

作　　者：李晨[1] 姜子涛[1] 李荣[1] 

机构地区：[1]天津商业大学生物技术与食品科学学院,天津市食品生物技术重点实验室,天津300134

出　　处：《食品科学》2013年第16期226-230,共5页Food Science

基　　金：天津市自然科学基金重点项目(120029);天津市高校科技发展基金计划项目(20110608)

摘　　要：采用高效液相色谱-串联质谱联用(HPLC-MS-MS)技术对樱桃叶中黄酮成分进行分离鉴别,流动相为0.1%甲酸溶液-甲醇溶液,梯度洗脱,在ESI负离子模式下采集数据,结合标准品对比,根据相对分子质量、质谱碎片等信息,从樱桃叶中鉴定出9种酚类成分,利用外标法及内标法对其进行定量,分别为:木犀草苷(14.42mg/g)、牡荆素(24.67mg/g)、芦丁(54.66mg/g)、金丝桃苷(56.74mg/g)、紫云英苷(44.20mg/g)、绿原酸(28.12mg/g)、奎宁酸(10.12mg/g)、槲皮素-3-芸香糖葡萄糖苷(20.15mg/g)、山奈酚-3-O-芸香糖苷(86.57mg/g)。建立的HPLC-MS-MS法快速有效可以用于鉴定樱桃叶中的化学成分。Flavonoids in the leaves of Prunus pseudocerasus L.were analyzed and identified by HPLC-MS-MS method.The mobile phase used for gradient elution was methanol/water containing 0.1% formic acid.Data acquirement was performed in the ESI negative ion mode.By comparison with authentic standards and based on details of molecular weight and mass spectral fragments,9 flavonoid components were identified and their quantitative determination was performed using external and internal standard methods,which were luteoloside（14.42 mg/g）,vitexin（24.67 mg/g）,rutin（54.66 mg/g）,hyperoside（56.74 mg/g）,astragalin（44.20 mg/g）,quinic acid（28.12 mg/g）,chlorogrnic acid（10.12 mg/g）,quercetin-3-rutinose-glucoside（20.15 mg/g）,kaempferol-3-O-rutinoside（86.57 mg/g）.The proposed method is rapid and reproducible and is useful to analyze flavonoids in the leaves of P.pseudocerasus.

关 键 词：樱桃叶 黄酮 高效液相色谱-串联质谱联用 山奈酚-3-O-芸香糖苷 

分 类 号：TS202.3[轻工技术与工程—食品科学]