氯化石蜡急性暴露对斑马鱼胚胎发育的毒性效应  

Toxicity effects on zebrafish (Danio rerio) larvae following embryonic acute exposure to chlorinated paraffins

作　　者：高永飞[1] 李佳[2] 李俊峰[2] 袁博[3] 张捷[1] 王亚韡[3] 梁勇[2,3] 

机构地区：[1]华中农业大学资源与环境学院,农业部亚热带农业环境国家重点实验室,武汉430070 [2]江汉大学医学院,武汉430056 [3]环境化学与生态毒理学国家重点实验室,中国科学院生态环境研究中心,北京100085

出　　处：《环境化学》2013年第8期1441-1447,共7页Environmental Chemistry

基　　金：国家973项目(2009CB421605);教育部新世纪优秀人才支持计划(NCET-11-0964)资助

摘　　要：比较研究了8种商品化氯化石蜡(CPs)急性暴露对斑马鱼(Danio rerio)胚胎早期发育的影响.实验结果表明,浓度分别为1、5、10和20 mg.L-1的CPs暴露7 d后均不会造成斑马鱼胚胎死亡,对斑马鱼胚胎的孵化率也无明显影响,但部分商品化CPs暴露对斑马鱼幼鱼鱼鳔发育具有明显的致畸效应,且随着暴露浓度的升高,鱼鳔畸形率显著上升,存在着明显的剂量-效应关系;此外,4种未氯化的短链石蜡烃(C10—C13)暴露对斑马鱼胚胎发育没有明显的毒性.上述研究结果表明,CPs急性暴露可造成斑马鱼幼鱼鱼鳔发育畸形,而这种致畸效应与CPs的含氯量不相关,与CPs中未经氯化的短链石蜡烃也无相关性,CPs对鱼类胚胎早期发育的潜在毒性效应需要进一步关注.This paper compares the effects of eight commercial chlorinated paraffins（CPs） on early embryonic development of zebrafish（Danio rerio） following embryonic acute exposure.The results showed that after 7 days exposure,CPs did not lead to embryonic mortality at the concentration of 1,5,10,20 mg.L-1.Meanwhile,no significant effects were observed on hatching rate among all groups,while gas bladders deformity was observed in zebrafish larvae exposed with some of the chlorinated paraffins.As the concentration of the CPs increased,deformity rate of gas bladders of zebrafish larvae raised,with an obvious dose-response correlation.Besides,four non-chlorinated short chain paraffins（C10—13） had no obvious toxicity on zebrafish embryonic development.These data indicated that CPs could cause deformity on the development of gas bladders of zebrafish larvae.However,the deformity effect on larvae had no relevance with either the chlorine content or the percentage of non-chlorinated short-chain paraffins in CPs.The potential toxicity of CPs on early embryonic development of fish needs further attention.

关 键 词：氯化石蜡 斑马鱼 胚胎 鱼鳔 

分 类 号：X174[环境科学与工程—环境科学]