乳腺癌p53基因突变规律及其预后意义  

Regular pattern and prognostic significance of p53 gene mutation in human breast cancer

作　　者：王洪义[1] 吕有勇[1] 金山 崔建涛[1] 何洛文[1] 徐光炜[1] 

机构地区：[1]北京医科大学临床肿瘤学院,北京市肿瘤防治研究所北京100036

出　　处：《实用肿瘤杂志》2000年第5期307-309,共3页Journal of Practical Oncology

摘　　要：目的　探讨 p5 3基因突变在乳腺癌发生发展中的演变规律及其与预后的关系。方法　应用 PCR-单链构象多态性 (PCR- SSCP)分析方法检测 5 5例原发性乳腺癌患者 p5 3基因 5～ 8外显子的突变情况。结果　 p5 3基因突变发生的频率为 36 .4% (2 0 / 5 5 ) , ～ 期和 ～ 期乳腺癌中 p5 3基因突变阳性率分别为 47.2 % (17/ 36 )和15 .8% (3/ 19) ,而且突变多发生在 5～ 6外显子。突变阳性患者其无病生存期明显较阴性者差 (P<0 .0 1) ,在 、 期乳腺癌中也以突变阳性患者的预后为差 (P<0 .0 5 )。结论　乳腺癌 p5 3基因突变多发生在病变早期 ,检测该基因的突变有可能成为判断 、 期乳腺癌预后的指标。Objective To study the clinical significance of p53 gene mutations associated with the development and prognostic significance in breast cancer.Methods PCR-single strand conformation polymorphism analysis (SSCP) was performed to detect the mutations of exon 5～8 in p53 gene of 55 patients with breast cancer.Results The total p53 gene mutation rate was 36.4%(20/55) and that was 47.2% in stage Ⅰ～Ⅱ and 15.8% in stage Ⅲ～Ⅳ.It was found that in this study the mutations of exon 5～6 in p53 gene were more common.The presence of p53 gene mutation was associated with a shortened disease-free survival in all patients (P<0.01),including those of stage Ⅰ～Ⅱ(P<0.05).Conclusion This observation indicates that p53 gene mutation may develop in earlier stage of breast tumorigenesis and it might be a useful prognostic indicator in patients with stage Ⅰ～Ⅱ breast cancer.

关 键 词：乳腺癌 预后 P53基因 基因突变 

分 类 号：R737.9[医药卫生—肿瘤]