聚丁二酸丁二醇酯降解菌株产脂肪酶条件的优化  

Optimization of Culture Conditions of Bacterium Producing PBS Degrading Lipase

作　　者：张敏[1,2] 沈颖辉 王旭愿[1] 李成涛[1] 吕嘉枥[1] 

机构地区：[1]陕西科技大学,轻化工助剂化学与技术教育部重点实验室,陕西西安710021 [2]浙江温州轻工研究院,浙江温州325003

出　　处：《环境科学与技术》2013年第8期29-33,共5页Environmental Science & Technology

基　　金：国家自然科学基金(21144008);温州科技计划项目(H20080045)

摘　　要：该研究采用Rashid N p-nPP比色法,通过单因素实验和正交实验对可生物降解聚丁二酸丁二醇酯(PBS)的铜绿假单胞菌产脂肪酶条件进行了优化。研究结果表明:最适培养条件为:温度29℃,摇床转速120 r/min;最佳培养基为:蔗糖0.5%,硫酸铵0.05%,硫酸亚铁0.005%,Tween-60与Span-80按1:1复配乳化剂0.5%,接种量5%,培养基初始pH 9.0。优化后酶活可达33.741 84 U/mL。Rashid N p-nPP colorimetric method was used to demine the lipase activity, which was produced by Pseudomonas aeruginosa cultured under optimized conditions through mono-factor and orthogonal experiments. Results show the optimized conditions of culture as 29 ℃ and rotating speed 120 m; while the optimal culture media is sucrose 0.5%, （NI-L）2SO4 0.05%, FeSO4 0.005%, compound emulsifier （Tween--60 ： Span-80 as i ： 1 ）0.5%, seed volume 5%, and the initial pH 9.0. After optimization,the lipase activity is up to 33.741 84 U/mL.

关 键 词：聚丁二酸丁二醇酯 铜绿假单胞菌 脂肪酶 条件优化 

分 类 号：X172[环境科学与工程—环境科学]