PML-RARα转基因小鼠发生急性早幼粒细胞白血病  

Development of Acute Promyelocytic Leukemia in PML-RARα Transgenic Mice

作　　者：孟秀琴 成国祥[2] 曹文俊[2] 诸江[2] 陈建泉 陈竺[2] 陈赛娟[2] 

机构地区：[1]上海铁路中心医院血液内科,上海200072 [2]上海第二医科大学附属瑞金医院,上海血液学研究所上海200025

出　　处：《中国实验血液学杂志》2000年第2期104-109,共6页Journal of Experimental Hematology

基　　金：上海血液学研究所胡应州基金部分资助

摘　　要：为了从整体水平上研究PML RARα融合蛋白的致白血病作用 ,建立了仅在髓系细胞中表达PML RARα融合基因的转基因小鼠。通过表型分析发现一个单系的 3只hCG PML RARα转基因小鼠在 1- 5个月时发生急性早幼粒细胞白血病 ,从而说明PML RARα融合基因的表达在白血病的发生中起着极为重要的作用。To investigate the leukemogenic potential of PML RARα fusion protein in vivo, hCG PML RARα transgene was constructed using molecular cloning technique and hCG PML RARα transgenic mice were generated. The genotype and phenotype of hCG PML RARα transgenic mice were analyzed by PCR, RT PCR, morphology of peripheral blood and bone marrow cells, and pathological examination of spleen, liver and bone marrow. As a result, acute promyelocytic leukemia was developed in 3 hCG PML RARα transgenic mice in 1-5 months. The results demonstrated that PML RARα fusion protein plays a crucial role in leukemogenesis.

关 键 词：PML-RARΑ融合基因 转基因小鼠 白血病 

分 类 号：R733.702[医药卫生—肿瘤]