羧甲基化虎奶多糖的制备及抗氧化性研究  

Preparation of Carboxymethylated Hunai Poly-saccharide and Study on Its Antioxidative Activities

作　　者：王雁[1] 杨祥良[1] 邓成华[1] 顾小曼 周井炎[1] 徐辉碧[1] 

机构地区：[1]华中理工大学药物研究所,武汉430074

出　　处：《生物化学与生物物理进展》2000年第4期411-414,共4页Progress In Biochemistry and Biophysics

摘　　要：通过对从虎奶菌菌核中提取的虎奶多糖 (HNP)进行羧甲基化修饰 ,制备了一种水溶性羧甲基化虎奶多糖 (CM HNP) ,并对其抗氧化性进行了初步研究 .结果表明CM HNP能有效抑制Fe2 + VitC引起的大鼠肝线粒体脂质过氧化、膜流动性的降低和线粒体的肿胀 ,清除邻苯三酚自氧化产生的超氧自由基O·2 并呈一定的剂量 效应关系 .A water soluble carboxymethylated derivative (CM HNP) of Hunai polysaccharide (HNP)from the sclerotia of Pleurotus tuber regium (Fr.)Sing. was prepared by the reaction of HNP with monochloroacetic acid. The antioxidative activities of CM HNP were also studied. The results showed that CM HNP can protect liver mitochondria from lipid peroxidation induced by Fe 2+ Vit C including the increase of TBARS contents, the swelling of mitochondria and the decrease of membrane fluidity with a dosage effect manner. In addition, CM HNP can effectively scavenge O · 2 generated by the self oxidation of pyrogallic acid.

关 键 词：虎奶多糖 抗氧化性 羧甲基化 

分 类 号：R282.71[医药卫生—中药学] TQ461[医药卫生—中医学]