染色体微切割、微克隆技术及其研究进展  

Techniques and Progress of Studies on Chromosome Microdissection and Microclone

作　　者：何聪芬[1,2] 辛志勇[1] 马有志[1] 

机构地区：[1]中国农业科学院作物育种栽培研究所,农业部作物遗传育种重点开放实验室 [2]河北师范大学生物系,石家庄050016

出　　处：《生物技术通报》2000年第1期26-32,共7页Biotechnology Bulletin

摘　　要：本文综述了染色体显微切割与微克隆技术的原理、方法、应用及研究进展，尤其对几种染色体显微切割的方法、染色体的体外扩增技术ＤＯＰ－ＰＣＲ、ＬＡ－ＰＣＲ等进行了比较分析。对染色体微切割、微克隆技术研究中存在的问题和应用前景进行了初步探讨。Several methods and development of studies on chromosomemicrodissection and microclone were summarized mainly in this paper. At thesame time, several methods of chromosome microdissection、DOP-PCR, LA-PCRetc. used in chromosome DNA amplification were comparatively analyzedrespectively, especially application prospects and problem of studies onchromosome microdissection and microclone were discussed.

关 键 词：染色体微切割 DNA扩增 微克隆 分子标记 

分 类 号：Q785[生物学—分子生物学]