NOX及细胞内ROS与胶质瘤增殖及恶性程度的关系  

Relationship of NOX and Intracellular ROS with Glioma Proliferation and Degree of Malignancy

作　　者：由丽娜 姜海东 

机构地区：[1]重庆市九龙坡区第一中医院,400080

出　　处：《实用癌症杂志》2013年第4期347-349,共3页The Practical Journal of Cancer

摘　　要：目的探讨NAD(P)H氧化酶(NOX)及其产物在胶质瘤组织中的表达以及两者与胶质瘤恶性程度的相关性。方法选取本院2011年1月至2013年1月经病理组织学证实为脑胶质瘤患者48例为研究对象,并选取同期行脑外伤手术的20例非肿瘤患者的正常脑组织为对照组。采用免疫组化法测定上述各组患者脑组织中NOX1～5 mRNA、NOX蛋白的阳性表达情况,并采用流式细胞仪测定各组患者脑组织中ROS的含量。结果与对照组相比,脑胶质瘤细胞组织中NOX1～5 mRNA以及ROS的含量显著增加,两两比较差异有统计学意义(P<0.05)。经单因素分析,NOX表达量与ROS的含量呈正相关(γ=0.698,P=0.001)。随着病理分级水平的增加,Nox表达阳性率显著增加,两两比较差异有统计学意义(P<0.05)。结论 NOX及细胞内ROS的含量与脑胶质瘤恶性程度有密切关系,两者可能在脑胶质瘤发生及发展过程中发挥重要的作用。Objective To investigate the expression NAD （P） H oxidase （NOX） and its product in glioma tissues and its correlation. Methods 48 patients confirmed by menstrual histopathology to be brain stromal tumors were selected and 20 ca- ses of normal brain tissue of non-cancer patients were selected as the control group. NOX1 - 5mRNA was detected by immuno- histochemistry and ROS levels in the brain tissue of patients was detected by flow cytometry. Results The brain stromal tumor cells NOX1 ~ 5mRNA and ROS significantly increased, the difference was statistically significant （P 〈 0.05 ）. NOX expression level and ROS content were positively correlated （T -- 0. 698 ,P = O. 001 ） by univariate analysis. With the increase in the level of pathological grade, the positive expression rate of NOX increased significantly, the difference was statistically significant （ P 0.05 ）. Conclusion NOX and intracellular ROS content glioma is closely related to the degree of malignancy, both of which may play an important role in occurrence and development of glioma.

关 键 词：活性酶族 脑胶质瘤 NAD(P)H氧化酶 

分 类 号：R739.41[医药卫生—肿瘤]