阿魏酸对大鼠离体胸主动脉环的舒张作用及其机制  

Vasodilatation effect and its mechanism of ferulic acid on isolated thoracic aortic rings from rats

作　　者：高秀艳[1] 宋士军[2] 崔同[3] 王腾腾[1] 李朝苹[1] 田粟[1] 

机构地区：[1]河北医科大学公共卫生学院营养与食品卫生学教研室,石家庄050017 [2]河北医科大学基础医学院生理学教研室,石家庄050017 [3]河北农业大学食品科技学院,保定071001

出　　处：《郑州大学学报（医学版）》2013年第4期459-463,共5页Journal of Zhengzhou University(Medical Sciences)

基　　金：河北省自然科学基金资助项目C2011204093;中国博士后科学基金资助项目20070410870

摘　　要：目的:探讨阿魏酸(FA)对大鼠离体胸主动脉环的舒张作用。方法:采用离体血管灌流法,检测FA对去氧肾上腺素(PE)和KCl预收缩大鼠离体胸主动脉环的舒张作用,观察去内皮或L-亚硝基精氨酸甲基酯(L-NAME)、亚甲蓝(MB)、吲哚美辛(Indo)、格列苯脲(Gli)、四乙基胺(TEA)孵育后舒张作用的变化;并观察在无钙K-H液中FA对PE诱发动脉环收缩作用的影响。结果:FA具有浓度依赖性地舒张PE和KCl预收缩的胸主动环的作用;完整内皮组和去内皮组的舒张作用的pD2分别为2.62±0.08和2.60±0.09(Z=0.447,P=0.655),与各种受体阻断剂孵育后FA的舒张作用间差异无统计学意义(P>0.05)。FA可明显抑制PE诱发的依外Ca2+性收缩反应(FFA=134.517,P<0.001)和依内Ca2+性收缩反应(F=108.816,P<0.001)。结论:FA具有舒张动脉环作用,其机制与抑制平滑肌细胞内Ca2+的释放和外Ca2+内流有关,其中考虑以抑制细胞内Ca2+的释放为主。Aim： To investigate the vasodilatation effects and mechanism of ferulic acid （ FA） on isolated thoracic aortic rings from rats.Methods： Using isolated vascular methods,the vasodilatation effects of accumulated FA on thoracic aortic rings preconstricted with L-phenylephrine hydrochloride （PE） and KCl were observed.After the isolated thoracic aortic rings were pretreated with removing the endothelium,or pretreated with the drugs,the changes of vasodilatation effects were observed.And in Ca2＋-free K-H solution,the influence of FA on thoracic aortic rings proconstricted with PE was observed.Results： With the increasing concentration,FA increased the vasodilatation effects of vascular aortic ringsin a dose-dependent manner.The values of pD 2 of endothelial groups and the endothelial-denuded group were 2.62±0.08, 2.60±0.93,respectively （Z=0.447,P=0.655） .Compared with them,there was no significant changes after incubation with the drugs of L-NAME,Indo,Gli,TEA,or MB （ P0.05） .In addition,FA inhibited PE-induced contraction in Ca2＋-free K-H solution（FFA=134.517,P0.001） .In Ca2＋-depleted,PE-treated aortic rings,FA inhibited the Ca2＋-induced contraction in a concentration-dependent manner（F=108.816,P0.001） .Conclusion： These results indicate that FA relaxes the thoracic aortic rings of rats in an endothelium-indepedended manner,and the vasorelaxing effect of FA might be mediated mainly through inhibiting Ca2＋release from intracellular stores and extracellular Ca2＋influx into vascular smooth muscle cells.

关 键 词：阿魏酸 胸主动脉环 舒张作用 机制 大鼠 

分 类 号：R151.1[医药卫生—营养与食品卫生学]