肺癌染色体8p21～23区域精细缺失图谱的构建  

Fine deletion mapping of chromosome 8p21～ 23 in lung cancer

作　　者：高志华[1] 李友军[1] 何春梅[1] 袁建辉 陈主初[1] 

机构地区：[1]湖南医科大学肿瘤研究所,湖南长沙410078

出　　处：《癌症》2000年第10期851-854,共4页Chinese Journal of Cancer

摘　　要：目的：进一步明确肺癌在 8p21～ 23区域等位基因杂合性丢失（ loss of heterozygosity, LOH）的频率与共同缺失区范围，以便分离该区域内与肺癌相关的候选抑瘤基因。方法：应用位于 8p21～ 23的 16个微卫星多态标记，对 32例肺癌组织和 2例肺癌细胞系进行 LOH分析。结果： 32例肺癌组织与 2例肺癌细胞系中共 20例（ 58.82％）存在至少一个位点的杂合性丢失，缺失频率最高的位点是 8p21.1的 NEFL与 D8s1771及 8p21.3的 D8s133，其频率分别为 45.16％（ 14/31）、 37.03％（ 11/27）和 35.72％（ 10/28）。 7例患者在 D8s1771～ D8s1477间存在连续性共同缺失。结论：肺癌在 8p21～ 23的最小共同缺失区位于 8p21的 D8s1771～ D8s1477之间，该区域可能存在一个与肺癌发生发展密切相关的抑瘤基因。Objective: To further determine the frequency and common deletion region of allelic losses on chromosome 8p21～ 23 in lung cancer and facilitate the isolation of the candidate tumor suppressor genes associated with lung cancer. Methods: Using 16 microsatellite markers, we mapped the frequently deleted region on 8p21～ 23 in 2 lung cancer cell lines (1 squamous cell carcinoma and 1 adenocarcinoma) and in 32 surgically resected primary lung tumors (3 small cell lung carcinomas, 13 squamous cell carcinomas, 11 adenocarcinomas and 5 adeno squamous cell carcinomas). Results: Overall, 20 of 34 (58.82％ ) samples showed allelic loss in at least one 8p locus. The most frequent losses of heterozygosity (LOH) occurred at the NEFL and D8s1771 loci (45.16％ , 37.03％ respectively) on chromosome 8p21.1 and at D8s133 locus (35.72％ ) on chromosome 8p21.3. Additionally, 7 cases were observed retaining a continuous allelic loss region between D8s1771 and D8s1477. Conclusion:These data show an interval of common deletion,flanked by D8s1771～ D8s1477 (about 1.5 centi Morgan), might localize a candidate tumor suppressor gene involved in lung carcinogenesis.

关 键 词：染色体8p21-23 杂合性丢失 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]