纳米硒对4-甲基亚硝胺-1-(3-吡啶)-1-丁酮诱发昆明小鼠肺癌的防治研究  

Nanoselenium prevents lung tumorigenesis induced by 4- (methylnitrosamino)-1-(3- pyridyl)-1-butanone in Kunming mice

作　　者：朱茂祥[1] 张劲松[2] 杨陟华[1] 方宇澄 龚诒芬[1] 高学云[2] 葛桂秀[1] 黄镇 

机构地区：[1]军事医学科学院放射医学研究所,北京100850 [2]中国科技大学经济技术学院,安徽合肥230052

出　　处：《癌症》2000年第10期883-886,共4页Chinese Journal of Cancer

摘　　要：目的：研究烟草特异亚硝胺 4甲基亚硝胺 1 (3吡啶 ) 1丁酮 [4 (methylnitrosamino) 1 (3 pyridyl) 1 butanone,NNK]诱发昆明小鼠发生肺癌及纳米硒对肺癌的防治作用。方法：用单次腹腔注射 NNK(500μ mol/kg)制成昆明小鼠肺癌模型，观察 8个月后肺癌发生率和肺肿瘤灶结节数，并进行病理诊断。在注射 NNK后第 1、 3、 7、 15和 30天测定腹腔巨噬细胞吞噬率、脾抗体生成细胞数和迟发型变态反应等免疫功能指标。结果：注射 NNK后 8个月，昆明小鼠肺癌发生率为 93.3％ (28/30)，肺肿瘤灶计数为 (5.075± 2.98)个 /肺，病理学诊断为腺癌。 NNK处理的小鼠分别饮用含硒 0.5、 1.0、和 3.0 ppm的纳米硒 (NS)，肺癌发生率和肺肿瘤灶计数分别下降到 69.2％ (18/26)、 56.6％ (17/30)、 46.7％ (14/30)和 (2.29± 2.23)、 (1.37± 2.47)、 (1.09± 1.31)个 /肺。在注射 NNK后的 1个月内，小鼠多种免疫功能出现不同程度的改变，而 NS对 NNK所致小鼠免疫功能失衡的调节作用明显。结论： NS对 NNK诱发小鼠肺癌有明显防治作用，并有较好的剂量效应关系，其机理可能与硒的免疫调节作用有关。Objective： To investigate whether nanoselenium(NS) can prevent lung tumorigenesis in Kunming mice induced by tobacco specific nitrosamine 4 (methylnitrosamino) 1 (3 pyridyl) 1 butanone(NNK). Methods： The model of NNK induced lung tumorigegesis in mice was established by a single dose of 500μ mol/kg intraperitoneally. The incidence of tumor and numbersof tumor nodes in the lung were observed 8 months after NNK treatment. The immune functions, such as phagocytosis of macrophage in the abdominal cavity, plaque forming cells and delayed type hypersensitivity etc. were examined at day 1, 3, 7, 15 and 30 following NNK injection. Results： Eight months after NNK treatment, the incidence of lung tumor in mice was 93.3％ (28/30), and the overage tumor nodes were 5.075± 2.98 per lung. The tumor was adenocarcinoma ascertained by histopathological examination. The 0.5, 1.0 and 3.0 ppm of selenium in NS decreased the incidence of tumor formation to 69.2％ (18/26), 56.6％ (17/30), 46.7％ (14/30) and reduced tumor nodes to 2.29± 2.23, 1.37± 2.47, 1.09± 1.31 per lung,respectively. The dysfunction of immune system in mice were observed during one month fouoming NNK administration, and were obviously ameliorated by NS. Conclusion： The NS can inhibit NNK induced lung tumorigenesis in mice with a better dose response relationship, and the mechanism may be related to immunoregulation of selenium.

关 键 词：NNK 肺肿瘤 免疫调节 纳米硒 小鼠 

分 类 号：R734.2[医药卫生—肿瘤]