蒙古蒿挥发油化学成分及其抑菌活性分析  

Analysis of Chemical Constituents of Volatile Oils from Artemisia mongolica and Their Antimicrobial Activities

作　　者：董岩[1] 祁伟[1] 肖传勇 

机构地区：[1]德州学院化学系,山东德州253023 [2]德州市农产品质量安全监督检测中心,山东德州253023

出　　处：《中国药学杂志》2013年第16期1359-1362,共4页Chinese Pharmaceutical Journal

基　　金：山东省自然科学基金资助项目(ZR2010BL015);德州市科技局科技攻关项目(2010A62-04)

摘　　要：目的用气相色谱-质谱对蒙古蒿中挥发油化学成分进行分析,并测定其挥发油的抑菌活性。方法采用CO2超临界萃取法提取蒙古蒿挥发油,用气相色谱-质谱联用技术(GC-MS)对蒙古蒿挥发油化学成分进行分离鉴定,用琼脂平板扩散法定量地测定蒙古蒿挥发油的抑菌活性。结果共分离出了28种化学成分,鉴定了23种成分;蒙古蒿挥发油对白色葡萄球菌、福氏志贺菌、乙型副伤寒杆菌均有明显抑制作用,对金黄色葡萄球菌无明显抑制作用。结论为蒙古蒿的进一步研究和开发应用提供理论依据。OBJECTIVE To analyze the chemical components of volatile oil from Artemisia mongolica by GC/MS, and to investigate their antimicrobial activities. METHODS The volatile oils were extracted from Artemisia mongolica by SFE-CO2 technique, their chemical components were analyzed by GC/MS, and their antimicrobial activities were tested by agar plate diffusion method. RE- SULTS Twenty-eight compounds were separated from the crude essential oils and 23 compounds were identified. The bacteriostatic experiment results indicated that this volatile oil has strong inhibitory effects on Staphyloccocus albus, Shigellaflexneri and Bacillus paratyphosus B, whereas it had no remarkable effect on Staphyloccocus aureus. CONCLUSION The experiment provides a scientific basis for further development and utilization of Artemisia mongolica.

关 键 词：蒙古蒿 挥发油 超临界萃取 抑菌性 气相色谱-质谱 

分 类 号：R284.2[医药卫生—中药学]