HPLC法测定苹果树腐烂病菌发酵液中的有机酸和葡萄糖  

Determination of organic acid and glucose in fermentation broth of Valsa mali Var. mali by high performance liquid chromatography

作　　者：简利茹[1] 王海瑛[2] 韩青梅[2] 

机构地区：[1]旱区作物逆境生物学国家重点实验室(西北农林科技大学),陕西杨凌712100 [2]西北农林科技大学植保学院,陕西杨凌712100

出　　处：《食品与发酵工业》2013年第7期204-207,共4页Food and Fermentation Industries

基　　金：陕西省科技统筹计划项目(2011KTZB02-02-02)

摘　　要：建立了一种用高效液相色谱（HPLC）检测苹果树腐烂病菌发酵液中有机酸和葡萄糖的方法。采用Atlantis C18色谱柱（150mm×4．6mm，3．5μm），以5g／LNH4H2PO4（用磷酸调pH=2．5）溶液为流动相，流速为0．6mL／min，柱温30℃，应用紫外检测器检测有机酸，示差检测器来分析葡萄糖。在此色谱条件下，葡萄糖和有机酸能很好地被分离检出，平均回收率为96．2％-102．2％，精密度RSD（n=5）为0．26％-0．75％，每一样品的分析时间不超过15min。该方法能够简便、快速测定苹果树腐烂病菌发酵体系中有机酸及葡萄糖的含量。A method for determination of organic acids and glucose in fermentation broth of Valsa mali var. mali by HPLC was established. The Atlantis C18 column （150 mm ×4.6 mm, 3.5 μm） was used. Mobile phase was 5 g/ L NH4H2PO4 （pH2.5）. The flow rate was 0.6 mL/min, and the temperature of column was 30℃. Organic acid and glucose in samples were determined by ultraviolet visible detector （UV） and refractive index detector （RID） , respectively. They were separated effectively at the chromatographic condition. The average recovery was in the range from 96.2% to 102.2% , and the relative standard deviation （ RSD, n =5） was in the range from 0.26% to 0.75%. By this method, organic acids and glucose can be easily, rapidly and effectively determined within 15 minutes.

关 键 词：HPLC 苹果树腐烂病菌 发酵液 有机酸 葡萄糖 

分 类 号：O657.72[理学—分析化学] S436.611[理学—化学]