基于响应曲面法优化桑黄菌深层发酵条件  

Optimization of submerged culture conditions of Phellinus linteus W-Ⅱ by response surface methodology

作　　者：崔凤杰[1] 王宏媛[1] 杨焱[2] 昝新艺[1] 孙文敬[1] 张志才[1] 黄达明[1] 

机构地区：[1]江苏大学食品与生物工程学院,镇江212013 [2]国家食用菌工程技术研究中心,上海市农业科学院食用菌研究所,上海204303

出　　处：《食品科技》2013年第8期61-66,共6页Food Science and Technology

基　　金：国家自然基金项目(31101269);国家"十二五"科技支撑计划项目(2012BAD36B05);中国博士后基金项目(2012M511702);江苏省2012年度普通高校研究生科研创新计划项目(CXLX12_0670);江苏大学"青年骨干教师培养工程"青年学术带头人培育计划项目;江苏省教育厅自然科学基金项目(07KJD350034)

摘　　要：采用单因素试验和响应面分析法优化桑黄W-Ⅱ深层发酵过程参数。单因素试验表明,玉米粉、麸皮+酵母膏和KH2PO4能显著影响桑黄菌丝体生物量。采用Box-Behnken试验进一步优化发现,以上各因素与桑黄菌丝体生物量存在显著的相关性,典型性分析结果表明,当培养基组成为玉米粉35.85g/L、麸皮16.52g/L、酵母膏3g/L、KH2PO41.0g/L时,桑黄菌丝体生物量达到最大,为10.68g/L。The mycelium biomass production from a wide strain Phellinus linteus W-Ⅱ was optimized by one-factor-at-a-time experiments and response surface methodology. Results showed that corn flour, bran and yeast extact, KH2PO4 significantly affected the mycelium biomass production. Further Box-Behnken design with response surface analysis proved that the above factors had the significant correlation with the response of mycelium biomass. The canonical analysis revealed that a maximum yield biomass of 10.68 g/L could be achieved at the concentrations point when corn flour, bran, yeast extract, and KH2PO4were set at 35.85 g/L, 16.52 g/L, 3 g/L and 1.0 g/L, respectively. Compared with the values obtained by other runs in the experimental design, the optimized medium resulted in a significant increase in the mycelial biomass production from the wide strain Phellinus linteus W-Ⅱ.

关 键 词：桑黄 野生菌 菌丝体 发酵培养 优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]