免疫亲和柱净化-HPLC法同时测定动物肌肉及牛奶中6种玉米赤霉醇类药物残留  

Simultaneous determination of residues of six kinds of zeranols in foodstuffs of animal origin by high performance liquid chromatography with immunoaffinity clean up column

作　　者：王清[1] 游丽娜[1] 李贤良[2] 郗存显[2] 黄亚[1] 唐柏彬[2] 张雷[2] 赵华[1] 

机构地区：[1]重庆医科大学药学院,重庆400016 [2]重庆出入境检验检疫局,重庆400020

出　　处：《食品工业科技》2013年第17期298-302,共5页Science and Technology of Food Industry

基　　金：科技部质检公益项目(201210086);国家认监委检验检疫行业标准项目(2011B138)

摘　　要：建立肌肉（猪肉、牛肉）及牛奶中6种玉米赤霉醇类化合物（α-玉米赤霉醇、β-玉米赤霉醇、α-玉米赤霉烯醇、β-玉米赤霉烯醇、玉米赤霉酮和玉米赤霉烯酮）残留量的免疫亲和柱净化-高效液相色谱同时检测方法。试样酶解后，肌肉样品经乙醚提取，牛奶样品经乙腈提取，免疫亲和柱净化，高效液相色谱仪测定。结果表明，6种目标物在0.01～0.2μg／mL范围内线性关系良好，相关系数（r）均大于0.999，检出限（S／N≥13）为1.0μg／kg，猪肉、牛肉、牛奶中的平均回收率分别在72.8％～88.4％、72.6％～92.4％、82.3％～102.3％之间，RSD均小于10.0％。该方法灵敏度高、重现性好，适用于猪肉、牛肉、牛奶等动物源性食品中痕量玉米赤霉醇类药物残留的测定。An immunoaffinity cleanup- high performance liquid chromatography （IAC- HPLC）method was established for the simultaneous determination of residues of six kinds of zeranols （co-zeranol, α-zeranol, β-zearalenol,α-zearalenol, zearalanone and zearalenone）in pork, beef and milk. Enzymolyzed samples were extracted with organic solvent （muscle by Ethyl ether, and milk by Acetonitrile）, subsequently cleaned up on a immunoaffinity column,and determined by HPLC.Six kinds of zeranols had good linear relationships in the range of 0.01～0.2μg/mL, the correlation coefficient was higher than 0.999, the limit of detection was 1.01μg/kg for each zeranol（S/N≥13）.Average recoveries of six target compounds in pork, beef and milk at different concentrations ranged was 72.8%～88.4% ,72.6%～92.4% and 82.3%～102.3%, relative standard deviation were less than 10.0%.The method could be used for determination of trace residues of six kinds of zeranols in foodstuffs of animal origin,and stable, reliable and accurate.

关 键 词：高效液相色谱法 玉米赤霉醇类 免疫亲和柱 动物肌肉 牛奶 

分 类 号：TS201.6[轻工技术与工程—食品科学]