复合酶酶解假酸浆黄酮苷抗氧化性研究  

Study on antioxidant activity of total flavonoids in nicandra physaloides extract

作　　者：孙维蔓[1] 王遂[1] 

机构地区：[1]哈尔滨师范大学化学化工学院,黑龙江哈尔滨150025

出　　处：《化学研究与应用》2013年第8期1195-1199,共5页Chemical Research and Application

基　　金：黑龙江省自然科学基金项目(B2009-04)资助

摘　　要：本文研究了复合酶酶解前后假酸浆提取物的抗氧化活性能力,并采用薄层色谱法(TLC)对酶解产物中总黄酮进行了分离,利用高效液相色谱-质谱联用技术对其鉴定分析。通过对DPPH自由基、羟自由基及超氧阴离子自由基清除能力实验,酶解液抗氧化性能显著高于假酸浆提取液;酶解液的薄层及HPLC-MS法初步鉴定出假酸浆中含有桑色素、二氢槲皮素和儿茶素三种黄酮。The enzymatic hydrolyzing condition was studied for flavonoids from Nicandra Physaloides.Flavonoid composition analysis was achieved by using TLC and HPLC-MS.According to experiment on free radical scavenging activity,that antioxidativity of samples incrdased in different degree after enzyme action.TLC and HPLC-MS shows four kinds of flavonoid aglycone：Morin、Taxifolin and Catechin.

关 键 词：假酸浆提取物 酶水解 抗氧化 薄层色谱法 高效液相色谱与质谱联用 

分 类 号：O629[理学—有机化学]