Fmr1基因敲除小鼠生后不同发育阶段的附睾iNOS的变化  

The iNOS expression of epididymis in Fmr1 knockout mice

作　　者：刘绪红 杨乙 颜仙姣 陈盛强[3] 孙卫文[3] 黄越玲[3] 戴丽军[3] 

机构地区：[1]龙岗区平湖人民医院,广东深圳518172 [2]深圳龙岗妇幼保健院,广东深圳518172 [3]广州医学院第二附属医院,广东广州510260

出　　处：《解剖学研究》2013年第4期241-245,共5页Anatomy Research

基　　金：广东省科技计划项目(2008B030301371);深圳市科技计划项目(201002161);深圳市龙岗区科技计划项目(YL-2009021)

摘　　要：目的对不同周龄的Fmr1基因敲除和野生型雄性小鼠附睾组织诱导型一氧化氮合酶(iNOS)的表达进行分析比较,探讨Fmr1基因敲除小鼠的附睾组织iNOS表达的差异,为脆性综合征的研究提供背景资料。方法本研究采用不同周龄(4、6、8、10周)的Fmr1(fragile X mental retardation 1)基因敲除型(knockout,KO)和野生型(wild-type,WT)各6只,先采用聚合酶链式反应(PCR)技术对KO小鼠和WT小鼠进行基因型鉴定,之后所有小鼠麻醉取附睾组织、石蜡包埋切片进行免疫组织化学染色技术对KO小鼠和WT小鼠附睾iNOS的表达进行检测并作对比分析。结果 iNOS在4周小鼠附睾阳性表达,在6、8和10周小鼠呈强阳性表达,且KO小鼠附睾的阳性表达均弱于WT小鼠。结论 Fmr1基因敲除小鼠在缺失Fmr1蛋白(fragile X retardation-1 protein,FMRP)后的附睾iNOS的表达降低。Objective The iNOS expression in different weeks of the Fmrl knockout and wild-type male mice epididymis tissue were analyzed and compared, to provide background information for the research of fragile X syndrome. Methods In this study, different weeks （4 weeks, 6 weeks, 8 weeks, 10 weeks） of the Fmrl gene knockout （KO） and wild-type （WT） of the six, first using the polymerase chain reaction （PCR） technique KO mice and WT mice genotyping, after which all mice testis tissue anesthesia, paraffin-embedded sections were immunohistochemical stained observation of of KO mice and WT mouse epididymis detect the expression of iNOS and for comparative analysis. Results The iNOS receptor in 4 weeks mice s positive expression, strongly positive expression in the 6 weeks, 8 weeks and 10 weeks mice, and KO mice were weaker positive expression of testis than WT mice. Conclusion The iNOS receptor expression in same week of the Fmrl knockout mice epididymis were significantly weaker than WT mice, suggesting that iNOS may be involved in the pathogenesis of fragile X syndrome.

关 键 词：脆性X综合征 小鼠 FMR1 附睾 一氧化氮 诱导型一氧化氮合酶 

分 类 号：R749.94[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]