儿茶素单体EGCG与ECG体外抗人肝癌细胞BEL-7402的作用  

Anti-tumor activities of catechins EGCG and ECG against human hepatocarcinoma BEL-7402 cells

作　　者：黄莉霞[1] 钟文彬[1] 黄树林[1] 胡耀华[1] 王丁丁[1] 孙艳[1] 

机构地区：[1]广东药学院生命科学与生物制药学院,广东广州510006

出　　处：《广东药学院学报》2013年第4期435-438,446,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金(81000923);广东省医学科学技术研究基金(B2009117);广东高校优秀青年创新人才培育项目(2009400)

摘　　要：目的研究儿茶素单体表没食子儿茶素没食子酸酯(EGCG)和表儿茶素没食子酸酯(ECG)抗人肝癌细胞BEL-7402的作用。方法形态学观察EGCG、ECG作用后BEL-7402细胞的生长;MTT法检测EGCG、ECG对BEL-7402细胞生长的抑制;流式细胞术(FCM)检测EGCG、ECG诱导BEL-7402细胞凋亡水平;RT-PCR检测增殖相关信号分子Gli2和凋亡受体Fas的表达水平。结果 EGCG、ECG均能以时间依赖和浓度依赖的方式抑制BEL-7402细胞生长,并诱导细胞凋亡。EGCG对BEL-7402细胞的生长抑制和诱导凋亡作用均较ECG强。EGCG和ECG均能下调Gli2的表达,但对Fas表达无影响。结论EGCG抗人肝癌BEL-7402细胞作用较ECG强。Objective To investigate the anti-tumor activities of Epigallocatechin gallate（EGCG） and Epicatechin gallate（ECG） against human hepatocarcinoma BEL-7402 cells.Methods Morphology of BEL7402 cells treated with EGCG or ECG was observed under microscope.Cell proliferation was assayed by MTT and growth inhibitory rate was calculated.Apoptosis of BEL-7402 was detected by flow cytometry.The expression of proliferation-related Gli2 and apoptosis receptor Fas genes was assayed by RT-PCR.Results Both EGCG and ECG inhibited cell growth and induced apoptosis of BEL-7402 cells in a timedependent and concentration-dependent manner.EGCG is more effective on cell growth inhibition and apoptosis induction than ECG.Both EGCG and ECG decreased Gli2 expression,but not for Fas expression.Conclusion The anti-tumor activity of EGCG against BEL-7402 cells is stronger than that of ECG.

关 键 词：表没食子儿茶素没食子酸酯 表儿茶素没食子酸酯 肝癌 抑制 凋亡 

分 类 号：R735.7[医药卫生—肿瘤]