γ-聚谷氨酸产生菌的诱变选育  

Mutation screening of γ-polyglutamic acid-producing strain

作　　者：王大芸[1,2] 曾伟 郑双凤[1,2] 贺杨扬[1,2] 陈桂光[1] 梁智群[1,2] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530004 [2]广西大学亚热带农业生物资源保护与利用国家重点实验室,广西南宁530004

出　　处：《中国酿造》2013年第8期25-29,共5页China Brewing

基　　金：国家自然科学基金项目(No.21062001);广西研究生教育创新计划资助项目(YCBZ2012004)

摘　　要：以Bacillus subtilis GXA-28为出发菌株,采用紫外、微波、氯化锂3种单一诱变及紫外-氯化锂,微波-氯化锂两种复合诱变方式进行诱变育种。经过5轮诱变,13次初筛,8次复筛,总共初筛选3554株,复筛选236株突变菌株。获得1株底物(谷氨酸钠)耐受性为出发菌株的2倍;γ-聚谷氨酸产量达24.00g/L左右(比出发菌株高出约33%)的菌株,命名为U-59。经过6代遗传稳定性试验,该菌株性状稳定。Bacillus subtilis GXA-28 was used as original γ-PGA-producing strain, which was treated by ultraviolet, microwave, lithium chloride, ultraviolet-lithium chloride and microwave-lithium chloride. After the mutagenesis, thirteen times screening, eight times rescreening, a total of 3554 screening, 236 rescreening, B. subtilis U59 was isolated. It was genetically stable. The substrate tolerance twice as original strain and the γ-PGA yield was attained 24.00g/L, increasing by 33%.

关 键 词：枯草芽孢杆菌 Γ-聚谷氨酸 底物耐受性 诱变育种 

分 类 号：Q815[生物学—生物工程]