Evaluation of Antitumor,Immunomodulatory and Free Radical Scavenging Effects of A New Herbal Prescription Seaweed Complex Preparation  

作　　者：LIU Xin SHAO Changlun KONG Wenwen FANG Yuchun WANG Changyun 

机构地区：[1]Key Laboratory of Marine Drugs,Ministry of Education,Marine Drug and Food Institute,Ocean University of China [2]Biology Institute of Shandong Academy of Sciences/Key Laboratory for Biosensors of Shandong Province

出　　处：《Journal of Ocean University of China》2013年第3期515-520,共6页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.30572314);the Basic Research Program of Science and Technology,Ministry of Science and Technology of China(No.2007FY210500);the Program of Chinese Offshore Investigation and Assessment,State Oceanic Administration of China(Nos.908-01-ST12,908-02-05-04);Science and Technology Program of Shandong Province,China(No.03BS109)

摘　　要：Seaweed Complex Preparation （SCP） is a clinical traditional Chinese medicine preparation which is composed of seven traditional Chinese herbs, and it has been used for treatment of lung cancer, liver cancer and digestive cancer. However, little infor- mation is available about the pharmacodynamic basis. The antitumor, immunomodulatory and free radical scavenging effects of SCP were evaluated in this study. Transplanted tumor in vivo method was used to determine the antitumor effect. The effects on spleno- cyte proliferation and phagocytosis of macrophages in tumor-bearing mice were measured by the MTT method and the phagocytizing cock red blood cell （CRBC） method respectively. The scavenging activities of SCP on DPPH and hydroxyl radicals in vitro were investigated. It was found that the medium-dose and high-dose of SCP could significantly inhibit the growth of transplanted hepatic tumor of murine hepatocarcinoma cell line H22, and promote proliferation of splenocytes and phagocytosis of macrophages. SCP possessed noticeable scavenging activities on DPPH and hydroxyl radicals. The antiturnor effects of SCP might be achieved by im- proving immune system and scavenging free radicals, which is in accordance with the viewpoint of traditional Chinese medicine in promoting the body resistance and eliminating pathogenic factors for cancer treatment.Seaweed Complex Preparation(SCP) is a clinical traditional Chinese medicine preparation which is composed of seven traditional Chinese herbs,and it has been used for treatment of lung cancer,liver cancer and digestive cancer.However,little information is available about the pharmacodynamic basis.The antitumor,immunomodulatory and free radical scavenging effects of SCP were evaluated in this study.Transplanted tumor in vivo method was used to determine the antitumor effect.The effects on splenocyte proliferation and phagocytosis of macrophages in tumor-bearing mice were measured by the MTT method and the phagocytizing cock red blood cell(CRBC) method respectively.The scavenging activities of SCP on DPPH and hydroxyl radicals in vitro were investigated.It was found that the medium-dose and high-dose of SCP could significantly inhibit the growth of transplanted hepatic tumor of murine hepatocarcinoma cell line H22,and promote proliferation of splenocytes and phagocytosis of macrophages.SCP possessed noticeable scavenging activities on DPPH and hydroxyl radicals.The antitumor effects of SCP might be achieved by improving immune system and scavenging free radicals,which is in accordance with the viewpoint of traditional Chinese medicine in promoting the body resistance and eliminating pathogenic factors for cancer treatment.

关 键 词：traditional Chinese medicine preparation Seaweed complex preparation ANTITUMOR IMMUNOMODULATORY free radicalscavenging 

分 类 号：R285[医药卫生—中药学]