溶藻细菌S7胞外溶藻活性物质的分离鉴定  

Purification and characterization of the extracellular algae-lysing components from an algae-lysing bacteria

作　　者：王金霞[1] 罗固源[2] 叶姜瑜[2] 许晓毅[2] 刘静[2] 

机构地区：[1]重庆工程职业技术学院矿业与环境工程学院,重庆400037 [2]重庆大学三峡库区生态环境教育部重点实验室,重庆400045

出　　处：《重庆大学学报（自然科学版）》2013年第9期141-145,共5页Journal of Chongqing University

基　　金：科技部国际合作项目(2007DFA90660);重庆市重大科技攻关项目(CSTC2006AA7003)

摘　　要：为了对菌株S7胞外溶藻活性物质进行分离鉴定，首先对溶藻细菌S7所分泌的溶藻活性物质进行分离和纯化，进而采用紫外光谱、红外光谱、荧光光谱和质谱对该活性物质进行了初步鉴定。结果表明，甲醇洗脱组分的溶藻效果最强，紫外光谱扫描结果显示溶藻细菌s7溶藻活性物质可能含有3～5个共轭的不饱和键；红外光谱扫描结果显示活性组分中可能含有羟基、饱和烃基和芳香醚；三维荧光光谱扫描结果显示溶藻活性物质有两个较强的荧光峰λex／λem，峰值分别出现在204／436nm和378／369nm处；质谱分析结果显示活性组分中含有6种物质，它们的分子量分别为163．1、246．3、274．3、318．3、404．5和475．4。Purification, characterization and initial identification of extracellular algae-lysing constituents of fermentation products of the algicidal bacteria S7 are carried out by ultraviolet spectrum, infrared spectrum, fluorescence spectra and mass spectrum, and the results show that the eluates of methanol have the best algicidal effects. These active algicidal substances made of conjugation unsatuated bonds, hydroxyl, saturated alkyl and aromatic ethers. In the three-dimensional fluorescence spectroscopy, two strong fluorescence peaks appear at 264/436 nm and 378/369 nm respectively. Mass spectrum indicates that the active substances consist of six monomers, and their molecular weights are 163. 1, 246. 3, 274. 3, 318.3, 404.5 and 475.4, respectively.

关 键 词：溶藻细菌 铜绿微囊藻 溶藻活性物质 纯化 鉴定 

分 类 号：X172[环境科学与工程—环境科学]