绿茶儿茶素对于人肝癌细胞的凋亡诱导作用  

Induction of Apoptosis in Human Hepatoma Cells by Green Tea Catechins

作　　者：刘术娟[1] 张乃鑫[2] 杨海贤[3] 褚建新 丁顺利 赵天如[2] 

机构地区：[1]天津市泌尿外科研究所 [2]天津医科大学病理学教研室,天津市300070 [3]天津医科大学电镜测试中心 [4]中国医学科学院血液学研究所实验病理室

出　　处：《中国肿瘤临床》2000年第12期902-905,共4页Chinese Journal of Clinical Oncology

基　　金：天津市自然科学基金!(编号97360941)

摘　　要：目的：探讨绿茶儿茶素（GTC）对BEL－7402人肝癌细胞的凋亡诱导作用。方法：应用透射电镜、流式细胞术、TUNEL和免疫组化等方法检测受100或200μg／ml GTC作用4天后的BEL－7402细胞的凋亡情况及其PCNA、bcl－2、c－myc、p53蛋白表达情况。结果：受GTC作用4天后的BEL－7402细胞的凋亡显著性增加，其PCNA表达显著性下降，而与凋亡相关的癌基因bcl－2、c－myc、p53蛋白表达未见明显改变。结论：GTC对于BEL－7402细胞的生长抑制作用可能与诱导细胞凋亡有关，也可能与抑制癌细胞DNA合成有关。本研究未发现GTC诱导BEL－7402细胞凋亡与bcl－2、c－myc、p53蛋白表达的相关性。Objective To probe the inductive effect of apoptosis of human BEL－7402 hepatoma cells by green tea catechins． Methods The human BEL－7402 hepatoma cells were incubated with 100 or 200ug／ml green tea catechins GTC for 4 days in vitro and apoptosis of these cells were studied by using transmission electron microscope flow cytometry and terminal deoxynucleotidyl transferase－mediated dUTP nick end labelling TUNEL and the expression of bcl－2 p53 c－myc as well as the proliferating cell nuclear antigen PCNA of BEL－7402 cells were also observed by using immunohistochemical staining． Results It showed that the apoptotic level of BEL－7402 cells significantly increased the expression of PCNA significantly decreased after incubated with 100 or 200ug／ml GTC for 4 days． No obvious differences were found in the expression of the three apoptosis－related genes bel－2 p53 and c－myc． Conclusion The results suggested that the growth inhibition of BEL－7402 cells by GTC might relate with the induction of apoptosis as well as the inhibition of DNA synthesis． There is no clearcut relationship between the expression of bcl－2 p53 and c－myc．

关 键 词：绿茶儿茶素 人肝癌细胞 细胞凋亡 大鼠 

分 类 号：R735.7[医药卫生—肿瘤] R73－361[医药卫生—临床医学]