化学发光酶免疫法测牛奶中3种喹诺酮类药物  

Determination of QNs Residues in Milk by Enhanced Chemiluminescent Enzyme Immunoassay

作　　者：李源珍[1] 生威[1] 刘恩梅[1] 韩静[1] 秦沛[1] 王硕[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院食品营养与安全省部共建教育部重点实验室,天津300457

出　　处：《食品研究与开发》2013年第16期78-81,共4页Food Research and Development

基　　金：天津市科技计划项目(10SYSYJC28300)

摘　　要：建立可同时检测牛奶中氧氟沙星（OFL）、麻保沙星（MAR）、氟罗沙星（FLE）残留的直接竞争化学发光酶免疫法（dc—CLEIA），方法的检出限分别为0．01、0．03、0．04ng／mL。牛奶中的氧氟沙星、麻保沙星、氟罗沙星用7．5％的三氯乙酸提取，测定200、100、50、20、10ng／mL5个添加水平的回收率，平均回收率在75．4％-94．1％之间。HPLC与dc—CLEIA测定的结果有很好的相关性，说明所建立的dc—CLEIA可用于实际样品的检测，结果准确可靠。A direct competitive enhanced chemiluminescent enzyme immunoassay （dc-CLEIA） for determination of ofloxacin （OFL）, marbofloxacin （MAR）, flerofloxacin （FLE） residues in milk was developed. The detection limit of assay were 0.01, 0.03, 0.04 ng/mL respectively. The spiked milk samples were extracted with trichloroacetic acid and analyzed by dc-CLEIA. The average recoveries at five spiked levels of 200, 100, 50, 20, 10 ng/mL were between 75.4 % and 94.1%. There was a good correlation between data obtained using the dc-CLEIA and HPLC, indicating the good performance of this dc-CLEIA. Therefore, the proposed method was simple, accurateand and suitable for the rapid determination of ofloxacin, marbofloxacin and flerofloxacin in milk samples.

关 键 词：氧氟沙星 麻保沙星 氟罗沙星 增强化学发光酶免疫法 牛奶 

分 类 号：R155.5[医药卫生—营养与食品卫生学]