中华青荚叶提取物体外抗氧化活性研究  

The Antioxidant Activities of Extracts In Vitro from Helwingia Chinensis

作　　者：唐健波[1,2] 姚佳[1,2] 肖雄[1,2] 杨娟[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002

出　　处：《山地农业生物学报》2013年第4期349-352,共4页Journal of Mountain Agriculture and Biology

基　　金：贵州省科技计划项目"贵州中药民族药研制创新能力建设"[院所创能(2009)]

摘　　要：采用80%乙醇对青荚叶进行提取,得到的醇提浸膏依次用石油醚、乙酸乙酯、正丁醇萃取。运用羟基(·OH)自由基体系、二苯代苦味基肼(DPPH·)自由基体系对Fe3+的还原能力进行试验,比较了中华青荚叶各提取物的抗氧化活性。结果表明:与抗坏血酸比较,中华青荚叶提取物对自由基均有较强清除作用,对·OH的清除率为乙酸乙酯萃取物>正丁醇萃取物>石油醚萃取物>水层部分>总醇提取物,IC50值分别为0.484、0.737、0.775、0.855、1.008 mg/mL;对DPPH·的清除率为乙酸乙酯萃取物>水层部分>正丁醇萃取物>总醇萃取物>石油醚萃取物,IC50值分别为0.047、0.060、0.061、0.073、0.721 mg/mL。各提取物对Fe3+均有较强的还原能力,其中正丁醇萃取物还原效果最为明显。Ethanol （80%）, petroleum ether, ethyl acetate and n-butanol were used to extract antioxidants from Helwingia chinensis leaves. The antioxidant activities of extracts in vitro were compared by experiments of the hydroxyl free radical （ . OH） , DPPH free radical （ DPPH. ） and deoxidization Fe^3 ＋ reaction system. Compared with the aseorbic acid, the extracts from Helwingia Chinensis showed stronger scavenging effects on the free radicals. The activity for scavenging . OH was in order of ethyl acetate extract 〉 n-butanol extract 〉 petroleum ether extract 〉 water extract 〉 total ethanol extract, whoes corresponding IC50 values were 0. 484 mg/mL, 0. 737 mg/mL, 0. 775 mg/mL, 0. 855 mg/mL and 1. 008 mg/mL, respectively. The DPPH--scavenging effect was in order of ethyl acetate extract 〉 water extract 〉 n-butanol extract 〉 total ethanol extract 〉 petroleum ether extract, and their corresponding IC50 values were 0. 047 mg/mL, 0.06 mg/mL, 0. 061 mg/ mL, 0. 073 mg/mL, 0. 721 mg/mL, respectively. All of the extracts demonstrated strong deoxidization ability to Fe^3＋ , among which the n-butanol extract ranked the first.

关 键 词：中华青荚叶 抗氧化 清除自由基 还原力 

分 类 号：Q946.3[生物学—植物学]