不同下颌前伸力对髁突Runx2和X型胶原表达的影响  

Effects of different modes of mandibular advancement on the Runx2 and Collagen X expression in condyle

作　　者：刘莹[1] 汤欢[1] 刘艳艳[2] 杨午[1] 刘畅[1] 

机构地区：[1]吉林大学口腔医院正畸科,长春130021 [2]吉林大学南岭校区医院口腔科

出　　处：《实用口腔医学杂志》2013年第5期621-625,共5页Journal of Practical Stomatology

基　　金：吉林省卫生厅资助项目(编号:2008Z010);吉林大学基本科研业务费-科学前沿与交叉学科创新项目(编号:200903225)

摘　　要：目的:探讨不同作用方式的下颌前伸力对髁突软骨内成骨的影响。方法:将28只5周龄雄性wistar大鼠随机分配到周期性、静止性、功能性下颌前伸组及阴性对照组(n=7)。各组按照设定的相关参数接受下颌前伸力刺激。实验结束时取标本,免疫组织化学染色,对Runx2、X型胶原的表达进行半定量分析。结果:Runx2和X型胶原表达动态组最高(与对照组比P<0.01),静态组和功能组较低(与对照组比P<0.01)。静态组X型胶原表达高于功能组(P<0.01),Runx2表达静态组与功能组差异无显著性(P>0.05)。结论:不同作用方式及作用时间的下颌前伸力差异性调节髁突软骨细胞Runx2及X型胶原的表达水平。Objective： To investigate the effects of different modes of mandibular advancement on endochondral ossification in con- dyle. Methods： 28 5-week-old male wistar rats were randomly divided into dynamic, static, functional and control groups （n = 7 ）, and received different modes of mandibular advancement according to their groups. After the experiment, sections of the condyles were pre- pared for immunohistochemistry staining, expression of Runx2 （runt-related transcription factor 2） and collagen X were semi-quantita- tively evaluated. Results： Runx2 and collagen X expression in dynamic group was higher, in static and functional group was lower than in the control （P 〈 0.01 ）. Between static and functional groups Runx2 expression was not significantly different （P 〉 0.05 ）. Col- lagen X expression in static group was higher than in functional group（ P 〈 0.01 ）. Conclusion： Different modes and duration of man- dibular advancement differently regulates the expression of Runx2 and collagen X in condylar chondrocytes.

关 键 词：周期性力 RUNX2 X型胶原 软骨内成骨 

分 类 号：R783.5[医药卫生—口腔医学]