微囊化胰岛素产生细胞移植治疗小鼠糖尿病的实验研究  

Study on the treatment of microencapsulated IPCs transplanted into diabetic mice

作　　者：穆长征[1] 蔡红玉[1] 李宏丹[1] 王雅光[1] 王恩达[1] 

机构地区：[1]辽宁医学院组织学与胚胎学教研室,辽宁锦州121001

出　　处：《中国现代医学杂志》2013年第22期12-15,共4页China Journal of Modern Medicine

基　　金：辽宁省自然基金(No:201102138)

摘　　要：目的探讨微囊化胰岛素产生细胞(IPCs)移植治疗糖尿病小鼠的疗效。方法应用大鼠胰腺提取物(RPE)诱导小鼠骨髓间充质干细胞(BMMSCs)分化为IPCs,采用免疫荧光鉴定细胞内胰岛素的表达。然后将其用海藻酸钠多聚赖氨酸(APA)微囊包裹起来。链尿佐菌素(STZ)腹腔注射制作糖尿病小鼠模型,随机分为3组:糖尿病模型组(对照组),微囊化IPCs移植组和未微囊化IPCs移植组。进行腹腔内移植。通过监测移植前后小鼠的血糖、胰岛素和C肽水平来观察治疗效果。结果糖尿病组小鼠血糖逐渐升高,未微囊化和微囊化组移植后均可改善糖尿病小鼠的高血糖状态,但未微囊化组只能维持血糖降低5 d左右,而微囊化组可持续降低血糖20 d以上,并且微囊化移植组的小鼠胰岛素和C肽水平明显升高。结论微囊化IPCs移植治疗小鼠糖尿病具有一定的效果,微囊具有较好的免疫隔离作用。[ Objective ] To explore the effect of microencapsulated insulin-producing cells （IPCs） transplanted into diabetic mice. [ Methods ] Rat pancreatic extract （RPE） was used to induce bone marrow mesenchymal stem cells （BMMSCs） into IPCs. The expression of insulin in IPCs was detected by immunofluoreseence. Then, the IPCs were wrapped up by alginate-polylysine-alginate （APA）. The diabetic mice model was made by intraperitoneal in- jection of streptozotocin. The diabetic mice were divided into three groups of random：control group （C）, nonmicroen- capsulated IPCs group （N） and microencapsulated IPCs group （M）. The IPCs were implanted into abdominal. The treatment effect was observed by monitoring the level of blood glucose, insulin and C peptide before and after trans- plantation. [ Results ] Blood glucose of diabetic mice （control group） gradually increased. Hyperglycemic state was both improved in group N and M after transplantation. The effect of reduced blood glucose could only maintain 5 days in group N, but that could maintain more than 20 days in group M. And the level of insulin and C peptide were increased obviously on the group M. [ Conclusion ] Microencapsulated IPCs had a certain effect in the treatment of diabetes. Microcapsule had better effect of immune isolation.

关 键 词：微囊化 骨髓间充质干细胞 胰岛素产生细胞 糖尿病小鼠 

分 类 号：R-332[医药卫生]