联合应用rhTNF和环磷酰胺治疗胶质瘤大鼠的效果及其机制  

Treatment effect of rhTNF combined with cyclophosphamide on glioma in rats and potential mechanism

作　　者：秦丽娟[1] 谷艳婷[2] 张田[1] 王艳蕾[1] 张一兵[1] 贾永森[3] 周洪霞[1] 张志勇[4] 薛一雪[5] 

机构地区：[1]河北联合大学基础医学院,河北省唐山市063000 [2]沈阳药科大学生命科学与生物制药学院生理学教研室,沈阳市110016 [3]河北联合大学中医学院,河北省唐山市063000 [4]河北联合大学附属医院,唐山市063000 [5]中国医科大学基础医学院神经生物学教研室,沈阳市110001

出　　处：《实用医学杂志》2013年第18期2977-2979,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:81101912);河北省卫生厅科研基金项目(编号:20120144);河北联合大学博士启动基金项目(编号:BS09012);河北联合大学大学生创新性实验计划项目(编号:X2012011);国家教育部新教师基金项目(编号:20102134120007);辽宁省博士启动基金项目(编号:20101109)

摘　　要：目的:探讨联合应用rhTNF和环磷酰胺治疗胶质瘤大鼠的效果及其可能机制。方法:制备胶质瘤大鼠模型,动态监测经颈总动脉给予rhTNF后的血肿瘤屏障开放程度。给予rhTNF 120 min再给予CTX后,ELISA法测定胶质瘤组织内CTX的含量。观察分别给予rhTNF、CTX和rhTNF(120 min)+CTX的胶质瘤大鼠的生存时间。结果:给胶质瘤大鼠注射rhTNF后,血肿瘤屏障开放程度增加,于120 min达最大后开始减小。给予rhTNF 120 min再给予CTX后,胶质瘤组织内CTX含量明显高于单独给予CTX组。与其他组相比,rhTNF(120 min)+CTX能明显延长胶质瘤大鼠的生存时间。结论:胶质瘤大鼠给予rhTNF 120 min时血肿瘤屏障开放程度最大,此时进入胶质瘤组织的CTX含量最多。Objective To investigate the effect of rhTNF combined with cyclophosphamide on glioma inrats and its possible mechanism. Methods Glioma-bearing rat models were established, dynamic monitoring was performed to indicate the extent of blood tumor barrier post rhTNF treatment through the carotid artery in glioma-bearing rats. CTX content of glioma tissue was detected by ELISA assay after treatment by rhTNF and CTX. Survival time was also observed in glioma-bearing rats receiving rhTNF or CTX or rhTNF plusCTX, respectively. Results The openness of blood-tumor barrier increased up to maximum at 120 min and then decreased after receiving rhTNF in glioma-bearing rats. CTX content of the glioma tissue was significantly higher than that in the CTX group. Compared with other groups, rhTNF （120 min） plus CTX treatment could significantly prolong the survival time of the glioma-bearing rats. Conclusion The blood-tumor barrier openness and CTX content in the glioma tissue could reach maximum after receiving rhTNF in glioma-bearing rats.

关 键 词：胶质瘤 血肿瘤屏障 RHTNF CTX 

分 类 号：R739.4[医药卫生—肿瘤]