中介素减轻大鼠高肺血流性肺血管结构重构  

Intermedin alleviates pulmonary vascular structural remodeling induced by high pulmonary blood flow in rats

作　　者：庞璐璐[1] 齐建光[1] 高扬[1] 金红芳[1] 杜军保[1] 

机构地区：[1]北京大学第一医院儿科,北京100034

出　　处：《基础医学与临床》2013年第10期1259-1263,共5页Basic and Clinical Medicine

基　　金：国家自然科学基金(30973226)

摘　　要：目的研究中介素(IMD)对大鼠高肺血流性肺血管结构重构的调节作用及其机制。方法雄性SD大鼠21只,随机分为对照组(n=7),分流组(n=8)和分流+IMD组(n=6)。对后两组大鼠行腹主动脉-下腔静脉分流术。8周后,对分流+IMD组大鼠,皮下埋微量渗透泵持续予IMD 1.5μg/(kg·h)。继续饲养2周后测定动脉平均压(mPAP)和右心室/(左心室+室间隔)重量比值,检测肺血管形态学变化,硝酸酶还原法测定血清和肺组织匀浆一氧化氮(NO)含量,Western blot方法测定肺组织内皮型一氧化氮合酶(eNOS)含量。结果与对照组相比,分流组大鼠mPAP、右心室肥厚程度、肺小血管肌化程度以及肺中、小动脉相对中膜厚度均明显增加,血清和肺组织匀浆NO含量明显降低,肺组织eNOS蛋白含量降低。IMD使肺动脉压力明显回降,右心室肥厚程度减轻,肺血管结构改变缓解,血清和肺组织匀浆NO水平以及肺组织eNOS蛋白含量均明显升高。结论 IMD可能通过eNOS/NO途径,减轻高肺血流性肺动脉高压和肺血管结构重构的形成。Objective To explore the regulating effect of intermedin （IMD） on the pulmonary vascular structural remodeling and pulmonary hypertension induced by high pulmonary blood flow in rats and its mechanism. Methods Twenty-one male SD rats were randomly divided into control group （n = 7 ）, shunt group （n = 8 ） and shunt with IMD group （n = 6 ）. Abdominal aorta and inferior vena cava shunting was performed in rats of the latter two groups. After 8 weeks, IMD [ 1.5 ~g/（ kg ~ h） ] was administered into rats of shunt with IMD group subcutaneous- ly by mini-osmotic pump for 2 weeks. Mean pulmonary artery pressure （mPAP） and the ratio of right ventricular mass to left ventricular plus septal mass [ RV/（ LV ＋ SP） ] was evaluated. The pulmonary vascular micro-morpho- logic changes of rats were observed. The content of NO in the serum and lung tissue homogenate was detected by nitric acid enzyme reduction method. The expression of eNOS protein in the lung tissue was detected by Western blot analysis. Results Compared with those of control group, mPAP, RV/（ LV ＋ SP） , the muscularization of small pulmonary vessels and relative medial thickness of pulmonary artery in rats of shunt group were all significantly increased. Meanwhile, the content of NO in the serum and pulmonary tissue homogenate and the expression of eNOS protein in rats of shunt group were all significantly decreased. However, IMD significantly decreased the mPAP and RV! （ LV ＋ SP）, alleviated the changes of pulmonary vascular micro-structure, with the elevation of the content of NO in the serum and pulmonary tissue homogenate and the expression of pulmonary eNOS protein in shunting rats. Conclusions IMD alleviates the development of pulmonary hypertension and pulmonary vascular structural remodeling induced by high pulmonary blood flow through eNOS/NO path way.

关 键 词：肺动脉高压 高肺血流 中介素 一氧化氮 

分 类 号：R363[医药卫生—病理学]