EGFR-siRNA对口腔鳞癌细胞耐药机制影响的研究  

Effect and Mechanism of EGFR-siRNA on the Resistant Cells of Oral Squamous Cell Carcinoma

作　　者：姚丛珊[1] 李德超[1] 朱杨[1] 赵艳宇[1] 齐嫦娥[1] 乎森[1] 

机构地区：[1]佳木斯大学口腔医学院颌面外科,黑龙江佳木斯154004

出　　处：《口腔医学研究》2013年第9期803-805,共3页Journal of Oral Science Research

基　　金：黑龙江省自然科学基金项目(D201025);佳木斯大学青年基金项目(Sq2011-021);黑龙江省人事厅留学回国人员科技项目择优资助基金(项目编号:黑人发[2009]23号)

摘　　要：目的:探讨EGFR-siRNA在人舌鳞癌细胞株顺铂耐药逆转过程中的作用。方法:逐步递增浓度、间歇作用体外诱导法建立Tca8113/CDDP细胞株。设计合成针对EGFR基因的siRNA干扰序列,瞬时转染Tca8113/CDDP细胞,采用RT-PCR法检测EGFR因子表达。MTT法测定顺铂对各组细胞的抑制作用。结果:EGFRsiRNA染效率达80%以上,各组细胞存活率存在浓度依赖,转染后相同浓度的顺铂作用后Tca8113/CDDP细胞株的抑制率明显升高(P<0.05),电泳带亮度明显弱于未转染组,EGFR mRNA表达下降(P<0.05)。结论:EGFRsiRNA能下调口腔鳞癌耐药细胞EGFR mRNA的表达,降低细胞增殖活性,并能恢复其对顺铂的敏感性。Objective： To demonstrate the effect of EGFR--siRNA on cisplatin resistent human tongue squamous cell carcinoma cells. Methods： Using gradually increasing and intermittent in--vitro induction of the drug ＂cis--di- amminedichloroplatinum （II） or cisplatin＂, we established Tca8113/CDDP cell line. We designed and synthesized siRNA sequence that interferes with EGFR gene, transfected it to Tca8113/CDDP cells, and measured the expres- sion of EGFR factor. Cells were divided into two groups, one was the drug resistent and the other was not. Two groups were exposed to different concentrations of the drug. The viability of cells was measured by MTT viability assay. Results： The efficiency of EGFR-- siRNA transfection was more than 80%. Cell viability was noticed to be a dose-- dependent. After exposing cells to the same concentration of cisplatin, inhibition rate for Tca8113/CDDP cell line was significantly higher （P〈0.05）, The electrophoresis bands showed weaker brightness than untransfect- ed control group. EGFR mRNA expression decreased （P〈0.05）. Conclusion： EGFR--siRNA can down--regulate the expression of EGFR mRNA in oral squamous cell carcinoma resistant cells, reduce cell proliferation activity and recover their sensitivity to cisplatin.

关 键 词：口腔鳞状细胞癌 顺铂 EGFR RNA 干扰 

分 类 号：R739.8[医药卫生—肿瘤]