低温纤维素降解菌的筛选及其产酶研究  

Study on screening and cellulase production of cold-adaped bacterium

作　　者：任红梅[1] 刘左军[1] 袁惠君[1] 水清照[1] 禄亚芬[1] 

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050

出　　处：《食品工业科技》2013年第19期127-130,134,共5页Science and Technology of Food Industry

基　　金：甘肃省自然科学基金(0710RJZA059);教育部全国大学生创新性实验计划项目(224002)

摘　　要：从甘南州玛曲县高寒草甸土壤中筛选出一株低温纤维素降解菌M7。通过形态观察、生理生化及16SrRNA序列比对分析,发现菌株M7与Bacillus subtili strain XS6进化距离最近,初步鉴定为枯草芽孢杆菌(Bacillus subtili)。通过Box-Benhnken中心组合设计和响应面分析法(RSM),对其生长和产羧甲基纤维素酶(CMCase)条件进行优化。结果表明:温度20℃,培养基初始pH5.0,转速175r/min,酵母膏2.5g/L和微晶纤维素2.5g/L,蛋白胨5g/L,菌株M7所产CMCase达到最大值。A strain M？ with cellulose degradation ability was screened from alpine meadow soil of Maqu county in Gansu province.It is identified as Bacillus subtilis by morphology, physiological and biochemical property and ]6SrRNA sequence analysis.The initial study on its producing cellulase showed that the temperature was 20℃, initial pHS.0, revolving speed l？Sr/min, peptone 5g/L, yeast extract 2.Sg/L and microcrystalline cellulose 2.5g/L. After the optimization of Box-Benhnken design and response surface methodology,the cellulase activity reached the maximum.

关 键 词：羧甲基纤维素酶 响应面 低温细菌 高寒草甸 优化 

分 类 号：TS201.3[轻工技术与工程—食品科学]