绿玉树茎皮脂溶性成分及其抗氧化活性研究  

The Liposoluble Components and the Antioxidant Activities from the Stems and Bark of Euphorbia tirucalli L

作　　者：李文基[1] 王茂媛[2] 陈烁[1] 张新蕊[1] 王建荣[2] 宴小霞[2] 王祝年[2] 

机构地区：[1]海南大学园艺园林学院,海南儋州571737 [2]中国热带农业科学院品种资源研究所农业部热带作物种质资源利用重点开放实验室,海南儋州571737

出　　处：《热带作物学报》2013年第9期1752-1755,共4页Chinese Journal of Tropical Crops

基　　金：农业部热带作物种质资源保护项目“南药种质资源保护”(No.13RZZY-16(2013));农业部财政专项“热带野生植物优异性状的鉴定与评价”(No.2130135)

摘　　要：运用气相色谱-质谱联用技术(GC-MS)对绿玉树茎皮脂溶性成分进行分析,为绿玉树的综合利用提供科学依据及数据支持。应用色谱峰面积归一化法定量分析各成分的相对百分含量,共分离鉴定出32个化合物,占其总量的96.01%。含量较高的分别是羊毛甾醇(29.86%)、13,27-环乌苏-3-酮(8.45%)、9,19-环羊毛甾-7-烯-3-醇(5.31%)。并运用DPPH法对绿玉树茎皮脂溶性成分进行抗氧化活性测定结果表明:其具一定的抗氧化活性,IC50为263.70μg/mL。The liposoluble components from the stems and bark of Euphorbia tirucalli L. were analyzed by GC- MS and the antioxidant activities were detected by DPPH free radical scavenging method. Thirty-two compounds were identified, amounting to 96.01% of the total detected constituents. The main chemical components of the liposoluble components were lanosterol（29.86%）, 13,27 -Cycloursan-3 -one （ 8.45% ）, 9,19-Cyclolanost-7-en-3-ol （5.31%）. The bioassay indicated that the liposoluble components from the stems and bark of E. tirucalli L. possessed antioxidant activity, with the IC5o value of 263.70 μg/mL.

关 键 词：绿玉树 气相色谱-质谱联用技术 抗氧化活性 

分 类 号：R284.2[医药卫生—中药学]