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作 者:惠伟[1] 谢欣蔚[1] 李彩香[1] 宋要强[1]
出 处:《西北农业学报》2013年第9期141-145,共5页Acta Agriculturae Boreali-occidentalis Sinica
基 金:陕西省农业科技创新项目(2012NKC01-22)
摘 要:为了探讨NO促进蒜苗生长的机制,用0.1、0.5和1.0μmol·L-1的NO气体熏蒸蒜种,测定蒜苗的生长指标和叶绿素荧光特性。结果表明,0.1、0.5μmol·L-1的NO气体熏蒸的蒜种可以促进大蒜生长,幼苗的最大叶片长、株高、假茎长、假茎粗都高于对照。前期研究表明,NO熏蒸大蒜促进蒜苗光合作用是非气孔因素起作用。以0.1和0.5μmol·L-1的NO气体熏蒸蒜种长成的蒜苗PSⅡ最大光化学效率(Fv/Fm)、PSⅡ实际光化学效率(Yield)、PSⅡ的潜在活性(Fv/Fo)、和光化学淬灭系数(qP)均明显大于对照;而初始荧光(Fo)和非光化学淬灭系数(NPQ)则低于对照和1.0μmol·L-1的NO气体处理。说明NO处理大蒜后对蒜苗生长的促进作用在于提高叶绿素的光化学活性。In order to explore the mechanism of nitric oxide (NO) promoting the growth of garlic seedling, the white garlic seeds were fumigated with 0.1 and 0.5 and 1.0 μmol·L-1 of NO, and growth indicators and chlorophyll fluorescence characteristics of garlic seedling were determined. The white garlic seeds fumigated with 0.1 and 0.5 μmol·L-1 of NO could promote the growth of garlic seedling. Max leaf length, plant height, pseudo stem length and pseudo stem width were higher than that of the control. Previous studies have shown that the light reaction ability of garlic seedlings was improved by exogenous NO. The maximal PSⅡ photochemical efficiency (Fv / Fm), the actual photochemical efficiency of PSⅡreaction center (Yield), PSⅡpotential activity (Fv / Fo), and photochemical quenching coefficent (qP) of the garlic seedlings from the garlic seeds that treated with 0.1 and 0.5 μmol·L-1 of NO gas were significantly higher than the control plants; Initial fluorescence (Fo) and non photochemical quenching coefficent (NPQ) was lower than that of the control and 1.0 μmol·L-1 NO gas treatment. The result showed that the growth promotion of NO treatment on garlic seedlings was caused by the increase of chlorophyll photochemical activity.
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