Eu-苦瓜多糖复合物的合成、抑制食道癌细胞增殖及与DNA相互作用  

Synthesis and Antiproliferative Effect on Esophagus Cancer Cells and Interaction with DNA of EuMomordica charantia L. Polysaccharide Complex

作　　者：张翠[1,2] 孙晓波[1] 宋爱荣[2] 潘维[1] 韩志建[1,2] 丁鲁军[1] 王进平[1,2] 

机构地区：[1]青岛农业大学化学与药学院,山东青岛266109 [2]青岛农业大学山东省应用真菌重点实验室,山东青岛266109

出　　处：《食品科学》2013年第17期327-332,共6页Food Science

摘　　要：目的：利用多糖提高机体免疫功能的抗肿瘤机理和稀土直接杀死、杀伤肿瘤细胞的药用价值，合成稀土-多糖复合物，发挥二者的协同作用，开发具有抗肿瘤作用的食品或保健品。方法：以苦瓜多糖为模板，均匀沉淀法制备稀土（Eu）-苦瓜多糖复合物（Eu-MCPS）；利用红外光谱、扫描电镜和透射电镜对其进行结构表征；噻唑蓝（MTT）比色法测定复合物对食道癌细胞的体外增殖抑制作用；光谱法研究Eu-MCPS与鲱鱼精脱氧核糖核酸（DNA）的相互作用。结果：稀土（Eu）以Eu（OH）CO3颗粒的形式附着在相互缠绕螺旋的多糖链上，并不通过化学键连接，Eu-MCPS形成，粒径分布在340～540nm。Eu-MCPS的质量浓度为100μg/mL时，对食管癌细胞的生长抑制率可达到21.1%，表明稀土-苦瓜多糖复合物对食管癌细胞具有一定的体外增殖抑制作用，并且光谱法证明稀土-苦瓜多糖复合物与DNA发生非嵌插作用。Objective： Polysaccharides have antitumor activity due to the stimulation to immune functions of human body, while rare earth elements have antitumor ability by killing tumor cells. In order to exploit food or healthcare products with antitumor effect, Eu-Momordica charantia L. polysaccharide complex （Eu-MCPS） was synthesized with the expectation of synergistic antitumor effects of the individuals. Methods： Eu-MCPS was synthesized with homogeneous precipitation by using Momordica charantia L. polysaccharide as the template. The complex was subjected to structural characterization employing FT-IR, scanning electron microscope and transmission electron microscope and its antitumor activity was evaluated by MTT assay. In addition, the interaction between Eu-MCPS and DNA was studied by UV-Vis spectroscopy and fluorescence spectroscopy. Results： Eu adhered to the twining and helical chains of Momordica charantia L. polysaccharide in the form of Eu（OH）CO3 particles instead of forming chemical bond with it. The particle size of Eu-MCPS varied from 340 nm to 540 nm. Eu-MCPS inhibited the proliferation of esophagus cancer cells, and its inhibitory rate was 21.1% at the concentration of 100 μg/mL. The interaction between Eu-MCPS and DNA may be a non-intercalation mode. These results can provide useful references to develop special functional foods or healthcare products.

关 键 词：多糖 稀土 稀土-苦瓜多糖复合物微粒 抗肿瘤 

分 类 号：TQ929.2[轻工技术与工程—发酵工程]