水相体系酶法制备甘油磷脂酰乙醇胺的研究  

Preparation of glycerylphosphorylethanolamine catalyzed by lipase

作　　者：张康逸[1,2] 康志敏[1] 范运乾[1] 王继红[1] 屈凌波[2] 

机构地区：[1]河南省农科院农副产品加工研究所,郑州450002 [2]郑州大学化学系,郑州450001

出　　处：《中国油脂》2013年第8期58-61,共4页China Oils and Fats

摘　　要：研究了水相体系中脂肪酶Thermo 4S-3水解大豆粉末磷脂制备甘油磷脂酰乙醇胺(L-α-GPE)的可行性,探讨了反应条件对磷脂酰乙醇胺(PE)转化率和L-α-GPE得率的影响。确定脂肪酶Thermo 4S-3催化制备L-α-GPE的最佳反应条件为:pH 7,底物质量浓度5 mg/mL,反应温度40℃,酶添加量30 U/mL,CaCl2添加量3.33 mg/mL,反应时间6 h。在最佳反应条件下,PE转化率为95.5%,L-α-GPE得率为93.6%。Thermo 4S - 3 lipase was applied to prepare L - alpha glycerylphosphorylethanolamine （L - a - GPE） by hydrolysis of soybean phospholipids powder in aqueous media. The effects of reaction conditions on the conversion rate of phosphorylethanolamine （ PE ） and the yield of L - α - GPE were discussed. The optimal reaction conditions were as follows： pH 7, mass concentration of substrate 5 mg/mL, reac- tion temperature 40 ℃, lipase dosage 30 U/mL, CaC12 dosage 3.33 mg/mL, reaction time 6 h. Under the optimal conditions, the conversion rate of PE and the yield of L - a - GPE reached 95.5% and 93.6%, respectively.

关 键 词：大豆粉末磷脂 磷脂酰乙醇胺 甘油磷脂酰乙醇胺 脂肪酶Thermo 4S-3 制备 

分 类 号：TQ464[化学工程—制药化工]