补充叶酸对脑梗死大鼠神经功能恢复的影响  

EFFECT OF FOLIC ACID SUPPLEMENTATION ON RECOVERY OF NERVE FUNCTION IN RATS WITH MIDDLE CEREBRAL ARTERY OCCLUSION

作　　者：曹家松[1] 秦善春[1] 张海红[1] 刘欢[1] 黄国伟[1] 

机构地区：[1]天津医科大学公共卫生学院营养与食品卫生学系,天津300070

出　　处：《营养学报》2013年第4期365-368,共4页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.81072289)

摘　　要：目的探讨叶酸干预对脑梗死大鼠脑脊液(cerebrospinal fluid,CSF)、血清叶酸及神经功能的影响。方法 40只SD大鼠随机分为四组:假手术组(Sham)、中动脉栓塞组(MCAO)、单纯叶酸补充组(Sham-FA)和叶酸补充中动脉栓塞组(FA-MCAO)。叶酸以0.8mg/kg bw·d灌胃,对照组给予等量生理盐水;4w后线栓法制备MCAO模型,术后1d及21d行Garcia JH评分;化学免疫发光法测定CSF及血清叶酸。结果 MCAO组大鼠梗死后CSF及血清叶酸均较造模前显著降低(P<0.05);叶酸干预(4w)下FA-MCAO组梗死前后CSF及血清叶酸均高于MCAO组(P<0.05),且脑梗死后CSF叶酸高于对照Sham-FA组,差异有统计学意义(P<0.05),FA-MCAO组的Garcia JH评分较MCAO组显著增加(P<0.05).结论脑梗死可降低SD大鼠CSF及血清叶酸水平。补充叶酸可显著提高并维持损伤后CSF叶酸高水平,促进神经功能恢复。Objective To investigate the dynamic changes ofcerebrospinal fluid （CSF） and serum folic acid and the neurological function in middle cerebral artery occlusion model rats with folic acid supplementation. Methods Forty SD rats stratified by weight were randomly divided into four groups： sham group （Sham）, middle cerebral artery occlusion group （MCAO）, simple folic acid supplemented group （Sham-FA） and folic acid supplemented plus middle cerebral artery occlusion group （FA-MCAO）. Sham-FA and FA-MCAO rats were intervened continuously by 0.8 mg/kgod bw doses of folic acid, and animals of control groups with same volume of saline. The left MCAO rats were modeled by thread occlusion method and neurological defects were scored in postoperative ld and 21d. The CSF and serum folic acid was determined by immuno chemiluminescence method. Results CSF and serum folic acid of MCAO rats was significantly decreased as compared to the normal （P〈0.05）. CSF and serum folic acid in FA-MCAO group （4w） was statistically increased, especially the CSF folic acid rapidly increased in FA-MCAO group compared with Sham-FA group （P〈0.05）. Garcia JH score was significantly increased in FA-MCAO group compared with MCAO group （P〈0.05）. Conclusion Ischemic brain injury of SD rats reduced CSF and serum folic acid level. Folic acid supplementation can effectively improve and maintain the high level of CSF folic acid and promote neurological function recovery after ischemic brain iujury.

关 键 词：叶酸 血清 脑脊液 脑梗死 中动脉栓塞 大鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]