内生短短芽胞杆菌011菌发酵滤液抑菌活性研究  

Antibiosis of the ferment of the endophyte Brevibacillus brevis strain 011

作　　者：王宇婷[1] 易有金[1,2,3] 夏菠[1] 杨建奎[4] 曾静[5] 

机构地区：[1]湖南农业大学食品科学技术学院,长沙410128 [2]湖南省食品科学与生物技术重点实验室,长沙410128 [3]湖南省发酵工程技术中心,长沙410128 [4]湖南农业大学理学院,长沙410128 [5]湖南农业大学园艺园林学院,长沙410128

出　　处：《植物保护》2013年第4期29-33,51,共6页Plant Protection

基　　金：国家自然科学基金项目(31071738;31000827);湖南省教育厅重点项目(10A051);湖南省自然科学基金项目(09JJ-3032);湖南农业大学稳定人才科学基金项目(07WDl8)

摘　　要：为明确短短芽胞杆菌011菌株在植物病害生物防治中的应用潜力,采用平皿打孔法测定011菌发酵滤液抗菌谱及其活性稳定性;采用孢子萌发法测定011菌发酵滤液对番茄早疫病菌最低抑菌浓度MIC及EC50;并显微观察其对番茄早疫病菌菌丝及孢子抑制作用。结果表明:011菌发酵滤液对8种植物病原真菌均有抑制作用,其中对番茄早疫病菌抑制作用较强,抑菌带宽度为5.5mm;对番茄早疫病菌菌丝及孢子均有致畸作用;对番茄早疫病菌最低抑菌浓度MIC为30%,有效抑菌中浓度EC50为9.64%;其活性具有较好的热稳定性(100℃处理1h活性保持在95%),耐酸性及抗紫外线(pH2.0处理及紫外20W,30cm照射120min,活性保持不变)。To investigate the utilization potential of the strain 011 of Brevibacillus brevis in biocontrol of plant dis eases, antibiosis spectrum and stability of its ferment were evaluated by plate-perforation method. MIC and EC50 of the ferment against Alternaria solani were determined by spore germination assay, and its inhibitive effects on both hyphae and spores of A. solani were validated by microscopy. The results suggested that the ferment of the strain 011 had an inhibitive effect on 8 fungal plant pathogens, and the strongest inhibition was observed in A. so lani, with a 5.5 mm width of inhibition band. Moreover, the ferment had a teratogenic effect on both hyphae and spores of A. solani ; the MIC and EC50 were 30% and 9.64%, respectively; the ferment had considerable heat sta bility （with 95% bioactivity reserved after treatment at 100 ℃ for 1 h）, acid tolerance and anti-UV property （bio activity was unaffected by pre-treatment at pH2.0 or 20 W UV radiation from a distance of 30 cm for 120 min）.

关 键 词：内生菌 抑菌活性 番茄早疫病菌 MIC EC50 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治]