破布木果总多酚的超声提取工艺研究  

Study on ultrasonic extraction technology of total polyphenols in Cordia dichotoma seeds

作　　者：刘枫[1] 张雪佳[2] 李洁[2] 田树革[2] 

机构地区：[1]新疆师范大学化学化工学院 [2]新疆医科大学中医学院中药系

出　　处：《环球中医药》2013年第8期580-584,共5页Global Traditional Chinese Medicine

基　　金：长江学者与创新团队专项基金(IRT0977)

摘　　要：目的采用正交试验法优化超声波辅助提取破布木果中总多酚的条件。方法在单因素实验的基础上,选择乙醇体积分数、料液比、提取时间、超声功率为随机因子,进行4因素3水平正交试验设计,采用SPSS软件分析了4个因素对总多酚得率的影响,并进行了验证试验。结果最佳提取工艺条件中乙醇浓度为30%（体积分数）,提取时间为30分钟,料液比（质量比）为1∶35,微波功率为400 W,没食子酸在质量浓度为1-7μg/ml（r=0.9995）时呈现良好的线性关系。验证试验提取率为（7.15 mg/g）比正交试验中最高提取率（7.01 mg/g）大。结论该提取方法操作简单,结果比较可靠,适用于破布木果总多酚的提取。Objective Orthogonal experimental method to optimize the ultrasonic extraction of the Cordia dichotoma seeds total polyphenols conditions.Methods On the basis of single factor experiments,select the ethanol concentration,solid-liquid ratio,extraction time,ultrasonic power as a random factor,the four factors and three levels of orthogonal experimental design using SPSS software to analyze the four factors on the yield of total polyphenols the impact,following by a verification test.Results The optimum extraction conditions in ethanol concentration of 70%（volume fraction）,the extraction time of 30 min,the solid-liquid ratio（mass ratio）1∶ 35,microwave power 400 W.Gallic acid showed a good linear relationship between the mass concentration of 1 ~ 7 μg / ml（r = 0.9995）.The extraction rate of the verification tests（7.15 mg / g）is bigger than the orthogonal tests of the highest extraction rate（7.01 mg / g）.Conclusions The extraction method is simple,the results are more reliable,and applicable to Cordia dichotoma seeds total polyphenol extraction.

关 键 词：破布木果 总多酚 超声提取 正交试验法 没食子酸 

分 类 号：R284.2[医药卫生—中药学]