海洋能源微藻的筛选及初步评价  

Screening and preliminary evaluation of oceanic microalgae for bioenergy production

作　　者：王能飞[1,2] 孙云飞[2,3] 王树春[2] 衣丹[2] 许宾[2] 王风芹[3] 臧家业[2] 

机构地区：[1]中国海洋大学海洋生命学院,山东青岛266100 [2]国家海洋局第一海洋研究所,山东青岛266061 [3]河南农业大学生命科学学院,河南郑州450002

出　　处：《化工进展》2013年第10期2366-2371,共6页Chemical Industry and Engineering Progress

基　　金：国家“十二五”科技计划(2011BAD14B04);中央级公益性科研院所基本科研业务费专项资金(2012G17/14);海洋公益性行业科研专项(201005031)项目

摘　　要：从黄海、南海部分海域采集水样,分离纯化了240株微藻,对其进行了分类与鉴定,从其中挑选8株生长较快的藻种进行扩大培养。经离心,冷冻干燥后得到藻粉,进而对藻种的淀粉、纤维素、总脂及可溶性糖含量进行了测量,以期筛选到目标能源微藻藻种。藻株Navicula sp.TW-2的油脂含量达到41.3%,生长速度快,生物量也较大,可以作为制备生物柴油的出发藻种。藻株Nannochloropsis sp.NB-3的纤维素含量达到9.26%,可溶性糖含量达到22.3%,生物量大,经过预处理发酵后生物乙醇产率可达7.75%,是制备生物燃料乙醇的优良藻株。The water samples were collected from south part of the Yellow Sea. Two hundred and forty microalgae were isolated and purified from the above samples using a capillary method. Among them, eight fast-growing algae strains were cultured in large scale. The lyophilized powder of the above algae cultures were prepared for the analysis of starch,cellulose,soluble sugar and total fat. One algae strain named Navicula sp. NB-2 yielded high dry biomass and total fat （413.3 mg/g dry biomass）,and therefore was selected as the initial strain for biodiesel production in future. Another stain named Navicula sp.NB-3 had fast-growing rate,high cellulose content （92.6 mg/g dry biomass） and high soluble sugar amount （223 mg/g dry biomass）. Moreover,Navicula sp.NB-3 yielded high bioethanol amount （77.5 mg/g dry biomass） and had potential for bioethanol production.

关 键 词：能源微藻 纤维素 可溶性多糖 生物乙醇 

分 类 号：TK6[动力工程及工程热物理—生物能]