AML-SCID小鼠模型的建立及鉴定  

Establishment and evaluation of AML-SCID mice model

作　　者：贾秀红[1] 张艳君 

机构地区：[1]滨州医学院附属医院儿科,滨州256603

出　　处：《滨州医学院学报》2013年第4期241-245,共5页Journal of Binzhou Medical University

基　　金：山东省科学技术发展计划项目(2010GSF10264);山东省自然科学基金资助项目(Y2007C018)

摘　　要：目的筛选出高表达HOXA9、HOXA10基因的急性髓系白血病(Acute myeloid leukemia,AML)细胞株,并建立AML模型,为研究HOXA9、HOXA10与AML的关系奠定基础。方法经RT-PCR检测HOXA9、HOXA10在U937、HL-60、NB4、Jurkat中的表达情况,筛选出高表达的细胞株;外周血瑞氏染色、骨髓流式细胞仪、脏器HE染色鉴定移植性U937人白血病重度联合免疫缺陷(Severe combined immunodeficiency,SCID)小鼠模型。结果 HOXA9、HOXA10在U937中均高表达,在HL-60、Jurkat中不表达(或表达水平较低),NB4高表达HOXA10;U937细胞在濒死小鼠骨髓细胞所占的比例为(20.62±9.66%)%,与对照组比较差异有统计学意义(P<0.05);病理检查发现U937细胞可浸润肝、脾、肺、肾器官。结论 U937高表达HOXA9、HOXA10,是研究HOXA9、HOXA10基因与白血病关系的合适细胞株,并成功建立U937-SCID人M5型急性髓性白血病模型。Objective To filter out the AML cell line with the HOXA9, HOXAIO gene high expression and establish the AML mod- el, so to lay the foundation for the study of the relationship between HOXA9, HOXAIO, and AML. Methods The expression of HOXA9 and HOXA10 mRNA in U937, HL-60, NB4, Jurkat were detected by reverse transcription PCR. A human leukemia model with severe combined immunedeficiency （ SCID） was intravenously inoculated with U937 cells. Peripheral blood cells were stained by Wright stain , flow cytometry instrument analyze the ration of CD45 positive cells in Bone marrow;organs were made into paraffin sections and then stained by Hematoxylin-eosin. Results HOXA9,HOXAIO mRNA level is high in U937 cells,whereas they cannot be detected in HL- 60 or Jurkat cells. HOXAIO mRNA level was high in NB4 cells. The proportion of human CD45 ＋ cells reached（ 20. 62 ±9. 66% ） % of bone marrow cells just prior to death of mice, which was different from the control group obviously （ P 〈 0. 05 ）. Pathological examina- tion revealed that U937 ceils could infihrate the organs of＇liver, spleen,lung and kidney. Conclusion U937 cell line is suitable for studying the relationship between HOXA9 （ or HOXA10 ） and leukemia. The U937-SCID mice was successfully established, which pro- vided a useful tool for researching in vivo.

关 键 词：HOXA9 HOXA10 U937 SCID BEIGE 白血病 

分 类 号：R733[医药卫生—肿瘤]